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DOI: http://dx.doi.org/10.11646/phytotaxa.218.2.3

Polulichloris henanensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga

huiyin song, qi zhang, guoxiang liu, zhengyu hu

Abstract


Coccoid green algae are abundant in subaerial habitats, but they are largely unexplored because of their morphological uniformity. Several new genus-level lineages have recently been described on the basis of molecular data. In this study, a coccoid green alga was isolated from surface soil in Zhoukou, Henan Province, China, and the cultured cells were described using light and electron microscopy. The ellipsoidal cell had smooth cell wall and parietal chloroplast with a pyrenoid surrounded by a starch envelope. Reproduction occurred by formation of 2‒16 autospores. Molecular phylogenetic analyses based on the nuclear 18S rDNA gene and the chloroplast ribulose-bisphosphate carboxylase gene (rbcL) indicated that this coccoid green alga represents a new lineage of the Watanabea clade (Trebouxiophyceae, Chlorophyta). Here, we describe this organism as a new genus and species, Polulichloris henanensis, gen. et sp. nov.


Keywords


Phylogeny, Taxonomy, Subaerial alage, Trebouxiophyceae, Watanabea clade, Algae

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References


Aboal, M. & Werner, O. (2011) Morphology, fine structure, life cycle and phylogenetic analysis of Phyllosiphon arisari, a siphonous parasitic green alga. European Journal of Phycology 46: 181‒192.

http://dx.doi.org/10.1080/09670262.2011.590902

Albertano, P., Pollio, A. & Taddei, R. (1991) Viridiella fridericiana (Chlorococcales, Chlorophyta), a new genus and species isolated from extremely acid environments. Phycologia 30: 346‒354.

http://dx.doi.org/10.2216/i0031-8884-30-4-346.1

Bock, C., Krienitz, L. & Proeschold, T. (2011) Taxonomic reassessment of the genus Chlorella (Trebouxiophyceae) using molecular signatures (barcodes), including description of seven new species. Fottea 11: 293‒312.

Darienko, T., Gustavs, L., Mudimu, O., Menendez, C.R., Schumann, R., Karsten, U. & Proschold, T. (2010) Chloroidium, a common terrestrial coccoid green alga previously assigned to Chlorella (Trebouxiophyceae, Chlorophyta). European Journal of Phycology 45: 79‒95.

http://dx.doi.org/10.1080/09670260903362820

Eliáš, M., Neustupa, J. & Škaloud, P. (2008) Elliptochloris bilobata var. corticola var. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccal green alga. Biologia 63: 791–798.

http://dx.doi.org/10.2478/s11756-008-0100-5

Eliáš, M., Němcová, Y., Škaloud, P., Neustupa, J., Kaufnerová, V. & Šejnohová, L. (2010) Hylodesmus singaporensis gen. et sp. nov., a new autosporic subaerial green alga (Scenedesmaceae, Chlorophyta) from Singapore. International Journal of Systematic and Evolutionary Microbiology 60: 1224‒1235.

http://dx.doi.org/10.1099/ijs.0.012963-0

Fawley, M.W., Yun, Y. & Qin, M. (2000) Phylogenetic analyses of 18S rDNA sequences reveal a new coccoid lineage of the Prasinophyceae (Chlorophyta). Journal of Phycology 36: 387‒393.

http://dx.doi.org/10.1046/j.1529-8817.2000.99105.x

Fučíková, K., Lewis, L.A. & Lewis, P.A. (2014) Widespread desert affiliation of trebouxiophycean algae (Trebouxiophyceae, Chlorophyta) including discovery of three new desert genera. Phycological Research 62: 294–305.

http://dx.doi.org/10.1111/pre.12062

Gaysina, L., Němcová, Y., Škaloud, P., Veselá, J., Ševčíková, T. & Eliáš, M. (2013) Chloropyrula uraliensis gen. et sp. nov.(Trebouxiophyceae, Chlorophyta), a new green coccoid alga with a unique ultrastructure, isolated from soil in South Urals. Journal of Systematics and Evolution 51: 476–484.

http://dx.doi.org/10.1111/jse.12014

Gerneck, R. (1907) Zur Kenntnis der niederen Chlorophyceen. Beihefte zum Botanischen Centralblatt 21 (2): 221–290.

Hanagata, N., Karube, I., Chihara, M. & Silva, P.C. (1998) Reconsideration of the taxonomy of ellipsoidal species of Chlorella (Trebouxiophyceae, Chlorophyta), with establishment of Watanabea gen. nov. Phycological Research 46: 221‒229.

http://dx.doi.org/10.1111/j.1440-1835.1998.tb00117.x

Huelsenbeck, J.P. & Ronquist, F. (2001) Mrbayes: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754‒755.

http://dx.doi.org/10.1093/bioinformatics/17.8.754

Karsten, U., Friedl, T., Schumann, R., Hoyer, K. & Lembcke, S. (2005) Mycosporine-like amino acids and phylogenies in green algae: Prasiola and ITS relatives from the Trebouxiophyceae (Chlorophyta). Journal of Phycology 41: 557‒566.

http://dx.doi.org/10.1111/j.1529-8817.2005.00081.x

Komárek, J. & Fott, B. (1983). Chlorophyceae (Grünalgen) Ordnung: Chlorococcales. In: Huber-Pestalozzi, G. (Ed.) Das Phytoplankton des Süßwassers 7. Teil, 1. Hälfte. Schweizerbart’sche Verlagsbuchhandlung (Nägele u. Obermiller), Stuttgart, pp. 1–1044.

Krienitz, L., Hegewald, E.H., Hepperle, D., Huss, V.A., Rohr, T. & Wolf, M. (2004) Phylogenetic relationship of Chlorella and Parachlorella gen. nov. (Chlorophyta, Trebouxiophyceae). Phycologia 43: 529‒542.

http://dx.doi.org/10.2216/i0031-8884-43-5-529.1

Krienitz, L., Hegewald, E., Hepperle, D. & Wolf, M. (2003) The systematics of coccoid green algae: 18S rRNA gene sequence data versus morphology. Biologia 58: 437‒446.

Kühn, J.G. (1878) Ueber eine neue parasitische Alge Phyllosiphon arisari welche die Laubblaetter einer terrestrischen Pflanze ganz in derselben Weise befaellt, wie dies von parasitischen Pilzen bekannt ist. Sitzungsberichten der naturforschenden Gesellschaft zu Halle 1878: 25‒26.

Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947‒2948.

http://dx.doi.org/10.1093/bioinformatics/btm404

Leliaert, F., Smith, D.R., Moreau, H., Herron, M.D., Verbruggen, H., Delwiche, C.F. & Clerck, O.D. (2012) Phylogeny and molecular evolution of the green algae. Critical Reviews in Plant Sciences 31: 1‒46.

http://dx.doi.org/10.1080/07352689.2011.615705

Leliaert, F., Verbruggen, H., Vanormelingen, P., Steen, F., Lopez-Bautista, J.M., Zuccarello, G.C. & De Clerck, O. (2014) DNA- based species delimitation in algae. European Journal of Phycology 49: 179–196.

http://dx.doi.org/10.1080/09670262.2014.904524

Luo, W., Proschold, T., Bock, C. & Krienitz, L. (2010) Generic concept in Chlorella-related coccoid green algae (Chlorophyta, Trebouxiophyceae). Plant Biology 12: 545‒553.

http://dx.doi.org/10.1111/j.1438-8677.2009.00221.x.

Ma, S., Huss, V.A., Tan, D., Sun, X., Chen, J., Xie, Y. & Zhang, J. (2013) A novel species in the genus Heveochlorella (Trebouxiophyceae, Chlorophyta) witnesses the evolution from an epiphytic into an endophytic lifestyle in tree-dwelling green algae. European Journal of Phycology 48: 200‒209.

http://dx.doi.org/10.1080/09670262.2013.790996

Medlin, L., Elwood, H.J., Stickel, S. & Sogin, M.L. (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA coding regions. Gene 71: 491–499.

http://dx.doi.org/10.1016/0378-1119(88)90066-2

Melkonian, M. (1990) Phylum Chlorophyta. Class Prasinophyceae. In: Margulis, L., Corliss, J.O., Melkonian, M. & Chapman, D.J. (Eds.) Handbook of Protoctista. Jones and Bartlett, Boston, pp. 608‒616.

Nadson, G. (1906) K morphologii nizshix vodoroslej Izvêstiya Imperatorskago S. Petersburgskago Botanicheskago Sada 6: 184–194.

Neustupa, J., Němcová, Y., Eliáš, M. & Škaloud, P. (2009) Kalinella bambusicola gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel coccoid Chlorella-like subaerial alga from Southeast Asia. Phycological Research 57: 159‒169.

http://dx.doi.org/10.1111/j.1440-1835.2009.00534.x

Neustupa, J., Eliáš, M., Škaloud, P., Němcová, Y. & Šejnohová, L. (2011) Xylochloris irregularis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga. Phycologia 50: 57‒66.

http://dx.doi.org/10.2216/08-64.1

Neustupa, J., Němcová, Y., Veselá, J., Steinová, J. & Škaloud, P. (2013a) Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two new Chlorella-like green microalgae (Trebouxiophyceae, Chlorophyta) from subaerial habitats. International Journal of Systematic and Evolutionary Microbiology 63: 377–387.

http://dx.doi.org/10.1099/ijs.0.047944-0

Neustupa, J., Němcová, Y., Veselá, J., Steinová, J. & Škaloud, P. (2013b) Parachloroidium gen. nov. (Trebouxiophyceae, Chlorophyta), a novel genus of coccoid green algae from subaerial corticolous biofilms. Phycologia 52: 411‒421.

http://dx.doi.org/10.2216/13-142.2

Nyati, S., Beck, A., & Honegger, R. (2007) Fine structure and phylogeny of green algal photobionts in the microfilamentous genus Psoroglaena (Verrucariaceae, lichen-forming Ascomycetes). Plant Biology 9: 390‒399.

http://dx.doi.org/10.1055/s-2006-924654

Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14: 817–818.

http://dx.doi.org/10.1093/bioinformatics/14.9.817

Rambaut, A. & Drummond, A. (2003) Tracer: MCMC tracer analysis tool. University of Oxford, Oxford.

Rodriguez, F., Oliver, J.L., Marin, A. & Medina, J.R. (1990) The general stochastic model of nucleotide substitution. Journal of Theoretical Biology 142: 485–501.

http://dx.doi.org/10.1016/S0022-5193(05)80104-3

Swofford, D.L. (2002) PAUP*. Phylogenetic analysis using Parsimony (*and other methods), version 4th ed. Sinauer Associates, Sunderland.

Zechman, F.W. (2003) Phylogeny of the Dasycladales (Chlorophyta, Ulvophyceae) based on analyses of RUBISCO large subunit (rbcL) gene sequences. Journal of Phycology 39: 819–827.

http://dx.doi.org/10.1046/j.1529-8817.2003.02183.x

Zhang, J., Huss, V.A., Sun, X., Chang, K. & Pang, D. (2008) Morphology and phylogenetic position of a trebouxiophycean green alga (Chlorophyta) growing on the rubber tree, Hevea brasiliensis, with the description of a new genus and species. European Journal of Phycology 43: 185‒193.

http://dx.doi.org/10.1080/09670260701718462


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