Open Access Open Access  Restricted Access Subscription or Fee Access


DOI: http://dx.doi.org/10.11646/phytotaxa.268.2.3

Hypnea wynnei and Hypnea yokoyana (Cystocloniaceae, Rhodophyta), two new species revealed by a DNA barcoding survey on the Brazilian coast

FABIO NAUER, VALÉRIA CASSANO, MARIANA C. OLIVEIRA

Abstract


Two new species of the red algal genus Hypnea (Gigartinales) are described for the southeastern coast of Brazil based on three molecular markers (COI-5P, rbcL and UPA), detailed morphological analysis, and comparison with closely related species. These species were named H. wynnei Nauer, Cassano & M.C. Oliveira and H. yokoyana Nauer, Cassano & M.C. Oliveira. Hypnea wynnei is characterized primarily by its reduced size, sub-erect tufts with few lateral branches and entangled basal portion, a thallus that is flattened only at the apical portions, and tetrasporangial sori developed only at apical portions of the branchlets. Hypnea yokoyana is characterized by its larger thallus, profuse branching, entirely terete axes, larger diameter of the main axes, and lack of entangling at its base. The molecular data support the conclusion that H. wynnei and H. yokoyana are distinct from other Hypnea species and are distantly related to Hypnea from the Brazilian coast.


Keywords


COI-5P, DNA barcode, Hypnea, molecular phylogeny, morphology, rbcL, UPA, Algae

Full Text:

PDF/A (1.54 MB)

References


Agardh, J.G. (1851) Species genera et ordines algarum, seu descriptiones succinctae specierum, generum et ordinum, quibus algarum regnum constituitur. Volumen secundum: algas florideas complectens. Part 2, fasc. 1. C.W.K. Gleerup, Lund, pp. 337–506.

Amado-Filho, G.M. (1991) Algas marinhas bentônicas do litoral de Saquarema a Itacoatiara (RJ). Dissertação de Mestrado. Universidade Federal do Rio de Janeiro. Rio de Janeiro, 323 pp.

Amado-Filho, G.M., Horta, P.A., Brasileiro, P.S., Barros-Barreto, M.B. & Fujii, M.T. (2006). Subtidal benthic marine algae of the Marine State Park of Laje de Santos (Sao Paulo, Brazil). Brazilian Journal of Oceanography 54: 225–234.

http://dx.doi.org/10.1590/S1679-87592006000300006

Chiang, Y.M. (1997) Species of Hypnea Lamouroux (Gigartinales, Rhodophyta) from Taiwan. In: Abbott, I.A. (Ed.) Taxonomy de Economic Seaweeds. Vol. 6. California Sea Grant College System, La Jolla, pp. 163–177.

Felsenstein, J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791.

http://dx.doi.org/10.2307/2408678

Fredericq, S. & Hommersand, M.H. (2003) Biogeography of the marine red algae of the South African West Coast: a molecular approach. Proceedings of the XVIIth International Seaweed Symposium 2003: 325–340.

Freshwater, D.W. & Rueness, J. (1994) Phylogenetic relationships of some European Gelidium (Gelidiales, Rhodophyta) species based on rbcL nucleotide sequence analysis. Phycologia 33: 187–94.

http://dx.doi.org/10.2216/i0031-8884-33-3-187.1

Geraldino, P.J.L., Yang, E.C. & Boo, S.M. (2006) Morphology and molecular phylogeny of Hypnea flexicaulis (Gigartinales, Rhodophyta) from Korea. Algae 21: 417–423.

http://dx.doi.org/10.4490/ALGAE.2006.21.4.417

Geraldino, P.J.L., Yang, E.C, Kim, M.S. & Boo, S.M. (2009) Systematics of Hypnea asiatica sp. nov. (Hypneaceae, Rhodophyta) based on morphology and nrDNA SSU, plastid rbcL, and mitochondrial cox1. Taxon 58: 606–616.

Geraldino, P.J.L., Riosmena-Rodriguez, R., Liao, L.M. & Boo, S.M. (2010) Phylogenetic relationships within the genus Hypnea (Gigartinales, Rhodophya), with a description of H. caespitosa sp. nov. Journal of Phycology 46: 336–345.

http://dx.doi.org/10.1111/j.1529-8817.2009.00804.x

Guiry, M.D. & Guiry, G.M. (2016) AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Available from: http://www.algaebase.org (accessed 20 June 2016)

Hall, T.A. (1999) BioEdit: a user-friendly biological alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Hommersand, M.H. & Fredericq, S. (2003) Biogeographycal of the marine red algae of the South African West Coast: a molecular approach. Proceedings of the Seventeenth International Seaweed Symposium 2003: 325–336.

Huelsenbeck, J.P. & Ronquist, F. (2001). MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17: 754–755.

http://dx.doi.org/10.1093/bioinformatics/17.8.754

Jesus, P.B., Guimarães, S.M.P.B. & Nunes, J.M.C. (2013a) Hypnea platyclada, a new species of red alga (Rhodophyta, Cystocloniaceae) from Brazil. Phytotaxa 85 (1): 26–34.

http://dx.doi.org/10.11646/phytotaxa.85.1.4

Jesus, P.B., Nauer, F., Lyra, G.M., Cassano, V., Oliveira, M.C., Nunes, J.M.C. & Schnadelbach, A.S. (2016) Species delimitation and phylogenetic analyses of some cosmopolitan species of Hypnea (Rhodophyta) reveal synonyms and misapplied names to H. cervicornis, including a new species from Brazil. Journal of Phycology. [Published online]

http://dx.doi.org/10.1111/jpy.12436

Jesus, P.B., Schnadelbach, A.S. & Nunes, J.M.C. (2013b) O gênero Hypnea (Cystocloniaceae, Rhodophyta) no litoral do estado da Bahia, Brasil. Sitientibus série Ciências Biológicas 13: 1–21.

http://dx.doi.org/10.13102/scb215

Jesus, P.B., Silva, M.S., Lyra, G.M., Nunes, J.M.C. & Schnadelbach, A.S. (2015) Extension of the distribution range of Hypnea stellulifera (Cystocloniaceae, Rhodophyta) to the South Atlantic: morphological and molecular evidence. Aquatic Botany 123: 26–36.

http://dx.doi.org/10.1016/j.aquabot.2014.12.003

Kützing, F.T. (1847) Tabulae phycologicae; oder, Abbildungen der Tange. Vol. I, fasc. 3–5. Gedruckt auf kosten des Verfassers, Nordhausen, pp. 27–36, pls. 21–50.

Lamouroux, J.V. (1813) Essai sur les genres de la famille des Thalassiophytes non articulées. Annales du Muséum d’Histoire Naturelle, Paris 20: 21–47, 115–139, 267–293, pls. 7–13.

Masuda, M., Yamagishi, Y., Chiang, Y.-M., Lewmanomont, K. & Xia, B. (1997) Overview of Hypnea (Rhodophyta, Hypneaceae). Taxonomy of Economic Seaweeds 6: 127–133.

Nauer, F., Cassano, V. & Oliveira, M.C. (2015) Description of Hypnea pseudomusciformis sp. nov., a new species based on molecular and morphological analyses, in the context of the H. musciformis complex (Gigartinales, Rhodophyta).
Journal of Applied Phycology 27: 2405–2417.

http://dx.doi.org/10.1007/s10811-014-0488-y

Nauer, F., Guimarães, N.R., Cassano, V., Yokoya, N.S. & Oliveira, M.C. (2014) Hypnea species (Gigartinales, Rhodophyta) from the southeastern coast of Brazil based on molecular studies complemented with morphological analyses, including descriptions of Hypnea edeniana sp. nov. and H. flava sp. nov. European Journal of Phycology 49: 550–575.

http://dx.doi.org/10.1080/09670262.2014.981589

Nunes, J.M.C. (2005) Rodoficeas marinhas bentonicas do estado da Bahia, Brasil, Tese de Doutorado. Instituto de Biociencias da Universidade de Sao Paulo. Departamento de Botanica. Sao Paulo, 410 pp.

Nunes, J.M.C., Moura, C.W.N., Figueiredo, M.A.O., Fujii, M.T., Cassano, V., Barros-Barreto, M.B.B. de, Pereira, S.M.B., Khader, S., Necchi Jr., O., Oliveira, M. C., Henriques, M.C., Oliveira-Carvalho, M.F., Guimarães, S.M.P.B., Costa, I.O., Lyra, G.M., Jesus, P.B., Tôrres, M.B. (2013) Rhodophyceae in Lista de Espécies da Flora do Brasil. Jardim Botânico do Rio de Janeiro. Available from: http://reflora.jbrj.gov.br/jabot/floradobrasil/FB100409 (accessed 1 July 2016)

Nylander, J.A.A. (2004) Bayesian phylogenetics and the evolution of gall wasps. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 2004: 1–34.

Ogawa, H. & Lewmanomont, K. (1981) Economic seaweeds of Thailand. I. The genus Hypnea in the vicinity of Si Racha, Chonburi Province. Kasetsart University Fishery Research Bulletin 12: 1–14.

Presting, G.G. (2006) Identification of conserved regions in the plastid genome—implications for DNA barcoding and biological function. Canadian Journal of Botany 84: 1434–1443.

http://dx.doi.org/10.1139/b06-117

Saito, R.S. & Oliveira, E.C. (1990) Chemical screening of Brazilian marine algae producing carrageenans. Hydrobiologia 204/205: 585–588.

http://dx.doi.org/10.1007/BF00040291

Saunders G.W. (2005) Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philosophical Transactions of the Royal Society B 360: 1879–1888.

http://dx.doi.org/10.1098/rstb.2005.1719

Saunders, G.W., Chiovitti, A. & Kraft, G.T. (2004) Small-subunit rDNA sequences from representatives of selected families of the Gigartinales and Rhodymeniales (Rhodophyta). 3. Delineating the Gigartinales sensu stricto. Canadian Journal of Botany 82: 43–74.

http://dx.doi.org/10.1098/rstb.2005.1719

Schenkman, R.P.F. (1986) Cultura de Hypnea (Rhodophyta) in vitro como subsídio para estudos morfológicos, reprodutivos e taxonômicos. Ph.D. thesis, Universidade de São Paulo, Brazil.

Scheneider, C.W. & Searles, R.B. (1976) North Carolina marine algae. VII. New species of Hypnea and Petroglossum (Rhodophyta, Gigartinales) and additional records of other Rhodophyta. Phycologia 15: 51–60.

http://dx.doi.org/10.2216/i0031-8884-15-1-51.1

Sherwood, A.R. & Presting, G.G. (2007) Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and cyanobacteria. Journal of Phycology 43: 605–608.

http://dx.doi.org/10.1111/j.1529-8817.2007.00341.x

Swofford, D.L. (2002) PAUP: Phylogenetic Analysis Using Parismony, version 4.08a. Computer program distributed by the Illinois Natural History Survey, Champaign, Ill.

Tanaka, T. (1941) The genus Hypnea from Japan. Scientific Papers of the Institute of Algological Research, Faculty of Science, Hokkaido University 2: 227–250.

Thiers, B. (2014) [continuously updated] Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. Available from: http://www.nybg.org/bsci/ih/ih.html (accessed 1 July 2016)

Villaça, R., Fonseca, A.C., Jensen, V.K. & Knoppers, B. (2010) Species composition and distribution of macroalgae on Atol das Rocas, Brazil, SW Atlantic. Botanica Marina 53: 113–122.

http://dx.doi.org/10.1515/BOT.2010.013

Wynne, M.J. (2011) A checklist of benthic marine algae of the tropical and subtropical western Atlantic: third revision. Nova Hedwigia Beiheft 140: 1–166.

Yamagishi, Y & Masuda, M. (2000) A taxonomic revision of a Hypnea charoides-valentiae complex (Rhodophyta, Gigartinales) in Japan, with a description of Hypnea flexicaulis sp. nov. Phycological Research 48: 27–35.

http://dx.doi.org/10.1046/j.1440-1835.2000.00181.x

Yamagishi, Y., Masuda, M., Abe, T., Uwai, S., Kogami, K., Kawagushi, S. & Phang, S.M. (2003) Taxonomic notes on marine algae from Malaysia. XI. Four species of Rhodophyceae 1. Botanica Marina 46: 534–547.

http://dx.doi.org/10.1515/BOT.2003.056

Yoneshigue, Y. (1985) Taxonomie et ecologie des algues marines dans la region de Cabo Frio (Rio de Janeiro, Bresil). PhD Thesis - Faculté dês Sciences de Lumin. L’Universite D’aix – Marseille I, 466 pp.


Refbacks

  • There are currently no refbacks.


ISSN 1179-3155 (print); ISSN 1179-3163 (online)

Published by Magnolia Press, Auckland, New Zealand