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DOI: http://dx.doi.org/10.11646/zootaxa.3768.3.2

One or two species? On the case of Hyperolius discodactylus Ahl, 1931 and H. alticola Ahl, 1931 (Anura: Hyperoliidae)

H. CHRISTOPH LIEDTKE, DOMINIK HÜGLI, J. MAXIMILIAN DEHLING, FABIO PUPIN, MICHELE MENEGON, ANDREW J. PLUMPTRE, DEO KUJIRAKWINJA, SIMON P. LOADER

Abstract


In 1931, Ernst Ahl described two species of reed frogs inhabiting montane forests of the Albertine Rift in East Africa, Hyperolius alticola and H. discodactylus, which were synonymized two decades later by Raymond Laurent. Since then, this revision has been questioned repeatedly, but taxonomists have been reluctant to make a conclusive decision on the matter, especially since the type material of H. alticola was reported as being lost. Here, we examine the rediscovered type material of H. alticola and reassess the validity of Laurent’s synonymy using morphological data from historic and new collections including all available type material, call recordings and molecular data from animals collected on recent expeditions. We find evidence for a northern and southern genetic clade, a divide that is somewhat supported by diverging morphology as well. However, no distinction in advertisement calls could be recovered to support this split and both genetic and morphological differences between geographic units are marginal and not always congruent and thus more likely reflect population-level variation. We therefore conclude that H. alticola is not a valid taxon and should continue to be treated as a synonym of H. discodactylus. Finally, we also report on newly collected material from outside the species known range, with first records of this species from Burundi.


Keywords


Albertine Rift, amphibian, morphology, phylogeny, 16S rRNA, reed frog, sexual dimorphism, advertisement call

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References


Ahl, E. (1931a) Das Tierreich. Eine Zusammenstellung und Kennzeichnung der rezenten Tierformen. Walter de Gruyter & Co., Berlin & Leipzig, 477 pp.

Ahl, E. (1931b) Zur Systematik der afrikanischen Artes der Baumfrochgattung Hyperolius. Mitteilungen aus dem Zoologischen Museum in Berlin, 17 (1), 1–132.

Barbour, T. & Loveridge, A. (1946) First supplement to typical reptiles and amphibians. Bulletin of the Museum of Comparative Zoology at Harvard College, 96 (2), 59–214.

Bell, R.C. & Zamudio, K.R. (2012) Sexual dichromatism in frogs: natural selection, sexual selection and unexpected diversity. Proceedings of the Royal Society B: Biological Sciences, 279, 4687–4693.
http://dx.doi.org/10.1098/rspb.2012.1609

Benson, D.A., Cavanaugh, M., Clark, K., Karsch-Mizrachi, I., Lipman, D.J., Ostell, J. & Sayers, E.W. (2013) GenBank. Nucleic Acids Research, 41, Database issue, 36–42.
http://dx.doi.org/10.1093/nar/gks1195

Channing, A. & Howell, K.M. (2006) Amphibians of East Africa. Comstock Publishing Associates, Cornell University Press, Ithaca, NY, 418 pp.

Channing, A., Hillers, A., Lotters, S., Rödel, M.-O., Schick, S., Conradie, W., Rödder, D., Mercurio, V., Wagner, P., Dehling, J.M., Preez, Du, L.H., Kielgast, J. & Burger, M. (2013) Taxonomy of the super-cryptic Hyperolius nasutus group of long reed frogs of Africa (Anura: Hyperoliidae), with descriptions of six new species. Zootaxa, 3620 (3), 301–350.
http://dx.doi.org/10.11646/zootaxa.3620.3.1

Conradie, W., Branch, W.R. & Tolley, K.A. (2013) Fifty Shades of Grey: giving colour to the poorly known Angolan Ashy reed frog (Hyperoliidae: Hyperolius cinereus), with the description of a new species. Zootaxa, 2636 (3), 201–223.

Conradie, W., Branch, W.R., Measey, G.J. & Tolley, K.A. (2012) A new species of Hyperolius Rapp, 1842 (Anura: Hyperoliidae) from the Serra da Chela mountains, south-western Angola. Zootaxa, 3269, 1–17.

Dehling, J.M. (2012a) An African glass frog: A new Hyperolius species (Anura: Hyperoliidae) from Nyungwe National Park, southern Rwanda. Zootaxa, 3391, 52–64.

Dehling, J.M. (2012b) Hyperolius discodactylus (Disc-Fingered Reed Frog). Parachuting. Herpetology Review, 43 (3), 463.

Dehling, J.M. & Matsui, M. (2013) A new species of Leptolalax (Anura: Megophryidae) from Gunung Mulu National Park, Sarawak, East Malaysia (Borneo). Zootaxa, 3670, 33–44.

Drewes, R.C. (1984) A phylogenetic analysis of the Hyperoliidae (Anura): treefrogs of Africa, Madagascar and the Seychelles Islands. Proceedings of the California Academy of Sciences, 139, 1–70.

Drewes, R.C. & Vindum, J.V. (1994) Amphibians of the Impenetrable Forest, southwest Uganda. Journal of African Zoology, 108, 55–70.

Drewes, R.C. & Wilkinson, J.A. (2004) The California Academy of Sciences Gulf of Guinea Expedition (2001) I. The Taxonomic Status of the Genus Nesionixalus Perret, 1976 (Anura: Hyperoliidae), Treefrogs of São Tomé and Príncipe, with Comments on the Genus Hyperolius. Proceedings of the California Academy of Sciences, 55, 1–13.

Duellman, W.E. (1970) The hylid frogs of Middle America. Volume 1. Monograph of the Museum of Natural History, University of Kansas, 1, 1–753.

Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (5), 1792–1797.
http://dx.doi.org/10.1093/nar/gkh340

Frost, D.R. (1985) Amphibian species of the world: a taxonomic and geographical reference. Association of Systematics Collection and Allen Press, Kansas, USA, 732 pp.

Frost, D.R. (2013) Amphibian Species of the World: an Online Reference. Version 5.6. American Museum of Natural History, NY, USA. Available from: http://researchamnhorg/herpetology/amphibia/indexhtml (accessed 26 August 2013)

Frost, D.R., Grant, T., Faivovich, J., Bain, R.H., Haas, A., Haddad, C.F.B., De Sa, R.O., Channing, A., Wilkinson, M. & Donnellan, S.C. (2006) The amphibian tree of life. Bulletin of the American Museum of Natural History, 297, 1–291.

Laurent, R.F. (1947) Sur quelque Rhacophorides nouveaux du Parc National Albert appartenant aux genres Megalixalus et Hyperolius. Bulletin de Musée Royal d'Histoire Naturelle de Belgique, 23 (7), 1–8.

Laurent, R.F. (1950) Exploration du Parc National Albert. Mission G. F. de Witte (1933-1935). Institut des Parcs Nationaux du Congo Belge, Brussels, Belgium, 149 pp.

Laurent, R.F. (1961) Notes sur les Hyperolius et quelques Afrixalus (Salientia) du Musée de Berlin. Revue de Zoologie et de Botanique Africaines, 64, 65–96.

Laurent, R.F. (1972) Exploration du Parc National des Virnga. Fondation pour favoriser les recherches scientifiques en Afrique, Bussels, Belgium, 125 pp.

Laurent, R.F. (1983) About the herpetofauna of Central African montane forest. In: Rhodin, A.G.J. & Miyata, K. (Eds.), Advances in hertpetology and evolutionary biology: essays in honor of Ernest E. Williams. Museum of Comparative Zoology, Cambridge MA, pp. 350–358.

Loader, S.P., Ceccarelli, F.S., Wilkinson, M., Menegon, M., Mariaux, J., de Sá, R.O., Howell, K.M. & Gower, D.J. (2013) Species boundaries and biogeography of East African torrent frogs of the genus Petropedetes (Amphibia: Anura: Petropeditidae). African Journal of Herpetology, 62 (1), 40–48.
http://dx.doi.org/10.1080/21564574.2013.781549

Loveridge, A. (1957) Check List of the Reptiles and Amphibians of East Africa (Uganda; Kenya; Tanganyika; Zanzibar). Bulletin of the Museum of Comparative Zoology at Harvard College, 117 (2), 1–362.

Masters, B.C., Fan, V. & Ross, H.A. (2011) Species Delimitation: a Geneious plugin for the exploration of species boundaries. Molecular Ecology Resources, 11 (1), 154–157.
http://dx.doi.org/10.1111/j.1755-0998.2010.02896.x

Murdoch, D.J. & Chow, E.D. (1996) A Graphical Display of Large Correlation Matrices. The American Statistician, 50 (2), 178–180.
http://dx.doi.org/10.1080/00031305.1996.10474371

Odierna, G., Aprea, G., Andreone, F., Böhme, W. & Vences, M. (2007) Cytosystematics of hyperoliid frogs: Phylogeny of Heterixalus, low karyotypic variability in hyperoliines and separate phylogenetic position of Leptopelis. Italian Journal of Zoology, 74 (1), 71–81.
http://dx.doi.org/10.1080/11250000601088564

Oksanen, J., Blanchet, F.G., Kindt, R., Legendre, P., Minchin, P.R., O’Hara, R.B., Simpson, G.L., Oksanen, M.J. & Suggests, M. (2013) Vegan: community ecology package. R package version 2.0-8.

Paepke, H.J. (2013) Ernst Ahl (1898 1945), sein Leben und seine Bedeutung für die Herpetologie und Terrarienkunde. Sekretär, 13 (1), 15–46.

Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (2002) The Simple Fool’s Guide to PCR, Version 2.0. Department of Zoology and Kewalo Marine Laboratory, Honolulu, Hawaii, 45 pp.

Plumptre, A.J., Davenport, T.R., Behangana, M., Kityo, R., Eilu, G., Ssegawa, P., Ewango, C., Meirte, D., Kahindo, C. & Herremans, M. (2007) The biodiversity of the Albertine Rift. Biological Conservation, 134 (2), 178–194.
http://dx.doi.org/10.1016/j.biocon.2006.08.021

R Core Team (2013) R: A language environment for statistical computing. http://wwwR-projectorg, R Foundation for statistical computing, Vienna, Austria.

Rambaut, A. & Drummond, A.J. (2007) Tracer version 1.4. Computer program and documentation distributed by the author. Available from: http://beastbioedacukTracer (accessed 26 August 2013)

Richards, C.M. & Moore, W.S. (1996) A phylogeny for the African treefrog family Hyperoliidae based on mitochondrial rDNA. Molecular Phylogenetics and Evolution, 5 (3), 522–532.
http://dx.doi.org/10.1006/mpev.1996.0047

Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Systematic Biology, 61 (3), 539–542.

Rödel, M.-O., Sandberger, L., Penner, J., Mané, Y. & Hillers, A. (2010) The taxonomic status of Hyperolius spatzi Ahl, 1931 and Hyperolius nitidulus Peters, 1875 (Amphibia: Anura: Hyperoliidae). Bonn Zoological Bulletin, 57, 177–188.

Savage, J. & Heyer, W. (1997) Digital webbing formulae for anurans: a refinement. Herpetological Review, 28 (3), 131.

Schick, S., Kielgast, J., Rödder, D., Muchai, V., Burger, M. & Lötters, S. (2010) New species of reed frog from the Congo basin with discussion of paraphyly in Cinnamon-belly reed frogs. Zootaxa, 2501, 23–36.

Schiøtz, A. (1975) The Treefrogs of Eastern Africa. Steenstrupia, Copenhagen, Denmark, 232 pp.

Schiøtz, A. (1999) Treefrogs of Africa. Edition Chimaira, Frankfurt (M), Germany, 350 pp.

Schiøtz, A & Drewes, R.C. (2004) Hyperolius discodactylus. In: IUCN 2013. IUCN Red List of Threatened Species. Version 2013.1. Available from: http://www.iucnredlist.org/details/56130/0 (accessed on 26 August 2013)

Schubotz, H. (1912) Wissenschaftliche Ergebnisse der Deutschen Zentral-Afrika-Expedition 1907-1908 unter führung Adolf Friedrichs, Herzog zu Mecklenburg. Band III Zoologie I. Klinkhardt und Biermann, Leipzig, 560 pp.

Tolley, K.A., De Villers, A.L., Cherry, M.I. & Measey, J.G. (2010) Isolation and high genetic diversity in dwarf mountain toads (Capensibufo) from South Africa. Biological Journal of the Linnean Society, 100 (4), 822–834.
http://dx.doi.org/10.1111/j.1095-8312.2010.01469.x

Veith, M., Kosuch, J., Rödel, M.-O., Hillers, A., Schmitz, A., Burger, M. & Lötters, S. (2009) Multiple evolution of sexual dichromatism in African reed frogs. Molecular Phylogenetics and Evolution, 51 (2), 388–393.
http://dx.doi.org/10.1016/j.ympev.2008.12.022

Venables, W.N. & Ripley, B.D. (2002) Modern applied statistics with S. Forth edition. Springer, New York, NY, 497 pp.

Vences, M., Kosuch, J., Glaw, F., Böhme, W. & Veith, M. (2003) Molecular phylogeny of hyperoliid treefrogs: biogeographic origin of Malagasy and Seychellean taxa and re-analysis of familial paraphyly. Journal of Zoological Systematics and Evolutionary Research, 41 (3), 205–215.
http://dx.doi.org/10.1046/j.1439-0469.2003.00205.x

Vences, M., Thomas, M., van der Meijden, A., Chiari, Y. & Vieites, D.R. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2 (5), 1–12.

Vonesh, J.R. (2005) Egg predation and predator-induced hatching plasticity in the African reed frog, Hyperolius spinigularis. Oikos, 110 (2), 241–252.
http://dx.doi.org/10.1111/j.0030-1299.2005.13759.x

Wieczorek, A.M., Drewes, R.C. & Channing, A. (2000) Biogeography and evolutionary history of Hyperolius species: application of molecular phylogeny. Journal of Biogeography, 27, 1231–1243.
http://dx.doi.org/10.1046/j.1365-2699.2000.00491.x


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