Open Access Open Access  Restricted Access Subscription or Fee Access


DOI: http://dx.doi.org/10.11646/phytotaxa.162.3.4

Suillus triacicularis sp. nov., a new species associated with Pinus roxburghii from northwestern Himalayas, India

Sudhakara M Reddy, Balwant Verma

Abstract


A new Suillus species, Suillus triacicularis sp. nov., is reported from northwestern Himalayan region of India. This is the first report of any Suillus species found in ectomycorrhizal association with Pinus roxburghii in this region. Morphologically, the species is very close to Suillus granulatus but can be distinguished mainly by the yellow to reddish or orange-yellow pileus color at maturity and the absence of watery green context next to the tubes. Phylogenetic analysis of internal transcribed spacer region revealed that this species is distinct from other closely related species of Suillus. Field photographs of fresh sporocarps, microscopic line drawings, and a phylogenetic tree are provided along with the taxonomic details.


Keywords


Ectomycorrhizal, ITS, Phylogeny, Suillaceae, Taxonomy

References


Arya, S., Kalia, R.K. & Arya I.D. (2000) Induction of somatic embryogenesis in Pinus roxburghii Sarg. Plant Cell Reports 19(8): 775–780.

http://dx.doi.org/10.1007/s002990000197

Baroni, T.J. (1978) Chemical spot-test reactions—Boletes. Mycologia 70(5): 1064-1076.

http://dx.doi.org/10.2307/3759138

Beatriz, R., Rincon, A.M., Maria, R.F. & Mercedes, F.P. (2006) Molecular characterization and evaluation of mycorrhizal capacity of Suillus isolates from Central Spain for the selection of fungal inoculants. Mycorrhiza 16: 465–474.

http://dx.doi.org/10.1007/s00572-006-0063-8

Bruns, T.D., Grubisha, L.C., Trappe, J.M., Kerekes, J.F. & Vellinga, E.C. (2010) Suillus quiescens, a new species commonly found in the spore bank in California and Oregon. Mycologia 102(2): 438–446.

http://dx.doi.org/10.3852/09-149

Corner E.J.H. (1972) Boletus in Malaysia. The Botanic Gardens, The Government Printer, Singapore, 263pp.

Dar, G.H., Beig, M.A. & Ganai, N.A. (2010) Influence of Ectomycorrhizal Inoculation on Blue Pine (Pinus wallchiania) and Deodar (Cedrus deodara) Seedlings. Trends in Biosciences 3(1): 60–62.

http://dx.doi.org/10.1111/j.1574-6941.2005.00014.x

Feng, J.-X., Wang, M.-J. & Yan, W. (2008) Study on molecular identification and genetic relationships of ectomycorrhizal strains of Suillus gene. Forest Research – Chinese Academy of Forestry 21: 825–831.

González-Ochoa, A.I., De las Heras, J., Torres, P. & Sánchez-Gómez, E. (2003) Mycorrhization of Pinus halepensis Mill. and Pinus pinaster Aiton seedlings in two commercial nurseries. Annals of Forest Science 60: 43–48.

http://dx.doi.org/10.1051/forest:2002072

Gray, S.F. (1821) A Natural Arrangement of British Plants. Baldwin, Cradock, and Joy, London, UK, p. 646.

Hall, T.A. (1999) Bio Edit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Jackson A.B. (1938) Pinus wallichiana In: Bulletin of Miscellaneous Information, Royal Botanic Gardens, Kew, Vol. 1938 No. 2. H.M. Stationery office, London, UK, p. 85.

Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780.

http://dx.doi.org/10.1093/molbev/mst010

Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Dictionary of the Fungi, 10th Eds. Wallingford, CABI, UK, p. 672.

Kornerup, A. & Wanscher, J.H. (1978) Methuen Handbook of Colour. Eyre Methuen Ltd., London, UK, 252pp.

Kretzer, A.M. & Bruns, T.D. (1997) Molecular revisitation of the genus Gastrosuillus. Mycologia 89: 586–589.

http://dx.doi.org/10.2307/3760995

Kretzer, A.M., Li, Y., Szaro, T. & Bruns, T.D. (1996) Internal transcribed spacer sequences from 38 recognized species of Suillus sensu lato: phylogenetic and taxonomic implications. Mycologia 88: 776–785.

http://dx.doi.org/10.2307/3760972

Kuntze, O. (1898) Revisio generum plantarum. A. Felix, Leipzig, Germany. 3: 1–576.

Loudon J.C. (1830) Loudon’s Hortus Britannicus, 1st eds. A. & R. Spottiswoode, New-Street-Square, London, UK, p. 388.

Manian, S., Sreenivasaprasad, S., Bending, G.D. & Mills, P.R. (2001) Genetic diversity and interrelationships among common European Suillus species based on ribosomal DNA sequences. FEMS Microbiology Letters 204: 117–121.

http://dx.doi.org/10.1111/j.1574-6968.2001.tb10873.x

Ronquist, F., Teslenko, M., Van-der Mark, P., Ayres, D., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2011) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology in press: 000–000.

Roussel, H.F.A. (1796) Flore du Calvados. L.-J. Poisson, Caen, France, 268 pp.

Sagar, A. & Lakhanpal, T.N. (2005) Pure culture synthesis of Pinus wallichiana ectomycorrhizal with Suillus sibiricus. Indian Phytopathology 58(3): 323–325.

Sargent C.S. (1897) The Silva of North America, Vol. XI. The Riverside Press, Cambridge, Massachusetts, USA, p. 9.

Sharma, K.R. & Lekha, C. (2013) Tapping of Pinus Roxburghii (Chir Pine) for Oleoresin in Himachal Pradesh, India. Advances in Forestry Letters 2(3): 53–57.

Singer, R. (1945b) The Boletineae of Florida with notes on extralimital species. II. The Boletaceae (Gyroporoideae). Farlowia 2(2): 223–303.

Smith, A.H. & Thiers, H.D. (1964) A contribution toward a monograph of the North American species of Suillus. The University of Michigan press, Ann Arbor, Michigan, USA, 116pp.

Thiers, H.D. (1979) The genus Suillus in the western united states. Mycotaxon 9: 285–296.

Timonen, S., Tammi, H. & Sen, R. (1997) Outcome of interactions between genets of two Suillus spp. and different Pinus sylvestris L. genotype combinations: identity and distribution of ectomycorrhiza and effects on early seedling growth in N-limited nursery soil. New Phytologist 137: 691–702.

http://dx.doi.org/10.1046/j.1469-8137.1997.00871.x

Wallander, H. (2000) Uptake of P from apatite by Pinus sylvestris seedlings colonized by different ectomycorrhizal fungi. Plant and Soil 218: 249–256.

http://dx.doi.org/10.1023/A:1014936217105

White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322.

Wu, Q.-X., Mueller, G., Lutzoni, F.M., Huang, Y.-Q. & Guo, S.-Y. (2000) Phylogenetic and biogeographic relationships of Eastern Asian and Eastern North American disjunct Suillus species (Fungi) as inferred from nuclear ribosomal RNA ITS sequences. Molecular Phylogenetics and Evolution 17: 37–47.

http://dx.doi.org/10.1006/mpev.2000.0812

Zhou, Z., Miwa, M. & Hogetsu, T. (1999) Analysis of genetic structure of a Suillus grevillei population in a Larix kaempferi stand by polymorphism of inter-simple sequence repeat (ISSR). New Phytologist 144: 55–63.

http://dx.doi.org/10.1046/j.1469-8137.1999.00504.x


Refbacks

  • There are currently no refbacks.


ISSN 1179-3155 (print); ISSN 1179-3163 (online)

Published by Magnolia Press, Auckland, New Zealand