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Late Quaternary Chrysophycean stomatocysts in a Southern Carpathian mountain lake, including the description of new forms (Romania)



In this study we present results of a low-resolution chrysophyte stomatocyst analysis that followed a high-resolution diatom analysis of a mountain lake sediment sequence from the Retezat Mountains, in the south Carpathians (Romania). The stomatocyst assemblages of the previously distinguished ten diatom assemblage zones of Lake Gales were studied with the aim to describe stomatocyst composition and create a taxonomical basis for detailed stratigraphical analysis in the future. We report 83 stomatocyst forms, and 7 of them are formally described here as new for science. An abrupt shift in cyst as well as diatom assemblages were recorded around 9200 cal yr BP during the 15,000 years long history of the Lake Gales. This Lake Gales event could be linked to the 9.3-ka widespread significant climatic anomaly, which was triggered by a melt water pulse into the North Atlantic.


Chrysophycean stomatocysts, climatic anomaly, diatoms, new morphotypes, 9.3 event, Retezat Mountains, Southern Carpathians

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Aboal, M., Alvarez Cobelas, M., Cambra, J. & Ector, L. (2003) Floristic list of non-marine diatoms (Bacillariophyceae) of Iberian Peninsula, Balearic Islands and Canary Islands. Updated taxonomy and bibliography. Diatom Monographs 4: 1–639.

Agardh, C.A. (1831) Conspectus Criticus Diatomacearum. Part 3. Lundae [Lund]: Literis Berlingianus. pp. 33–48.

Battarbee, R.W. (1986) Diatom analysis. In: Berglund, B.E. (ed.) Handbook of Holocene Palaeoecology and Palaeohydrology. John Wiley & Sons, Chichester, New York, Brisbane, Toronto, Singapore, pp. 527–570.

Baumann, E., de Jong, R., & Kamenik, C. (2010) A description of sedimentary chrysophyte stomatocysts from high-Alpine Lake Silvaplana (Switzerland). Nova Hedwigia, Beiheft 136: 71–86.

Betts-Piper, A.M., Zeeb, B.A. & Smol, J.P. (2004) Distribution and Autecology of Chrysophyte Cysts from High Arctic Svalbard Lakes: Preliminary Evidence of Recent Environmental Change. Journal of Paleolimnology 31: 467–481.

Braun, M., Hubay, K., Magyari, E.K., Veres, D., Papp, I. & Bálint, M. (2013) Using linear discriminant analysis (LDA) of bulk sediment geochemistry data to reconstruct Lateglacial climate change in the South Carpathian Mts. Quaternary International 293: 114–122.

Buczkó, K. (2004) Szent Anna cisztái. [Stomatocysts of Lake Saint Anna]. Élet és Tudomány 59: 748–750.

Buczkó, K. & Magyari, E. (2006) A Szent Anna-tó a palaeolimnológus szemével. [The Lake Saint Anna in the palaeolimnologists eyes]. Természet Világa 137: 570–571.

Buczkó, K., Magyari, E., Soróczki-Pintér, É., Hubay, K., Braun, M. & Bálint, M. (2009) Diatom-based evidence for abrupt climate changes during the Lateglacial in the South Carpathian Mountains. Central European Geology 52: 249–268.

Buczkó, K., Magyari, E.K., Hübener, T., Braun, M., Bálint, M., Tóth, M. & Lotter, A.F. (2012) Responses of diatoms to the Younger Dryas climatic reversal in a South Carpathian mountain lake (Romania). Journal of Paleolimnology 48: 417–431.

Buczkó, K., Magyari, E.K., Braun, M. & Bálint, M. (2013) Diatom˗inferred lateglacial and Holocene climatic variability in the South Carpathian Mountains (Romania). Quaternary International 293: 123–135.

Bukhtiyarova, L. & Round, F.E. (1996) Revision of the genus Achnanthes sensu lato section Marginulatae Bukh. sect. nov. of Achnanthidium Kütz. Diatom Research 11: 1–30.

Cabała, J. & Piàtek, M. (2004) Chrysophycean stomatocysts from the Staw Toporowy Nizni lake (Tatra National Park, Poland). Annales de Limnologie 40: 149–165.

Cabała, J. (2005) Chrysophycean stomatocysts from Morskie Oko and Żabie Oko lakes in the Tatra National Park, Poland. Acta Societatis Botanicorum Poloniae 74: 305–314.

Cărăuş, I. (2012) Algae of Romania—a distributional checklist of acutal algae. In: Studii şi Cercetări Ştiinţifice, Seria Biologie, Universitatea din Bacău, Facultatea de Ştiinţe, Catedra de Biologie, 2002, 809 pp., version 2.3—third revision

Cleve, P.T. & Grunow, A. (1880) Beiträge zur kenntniss der arctischen Diatomeen. Kongliga Svenska-Vetenskaps Akademiens Handlingar 17: 1–121.

Czarnecki, D.B. (1994) The freshwater diatoms culture collection at Loras College, Dubuque, Iowa. In: Kociolek, J.P. (ed.) Proceedings of the 11th International Diatom Symposium, Memoirs of the California Academy of Sciences. San Francisco. pp. 155–174.

Cronberg, G. & Sandgren, C.D. (1986) A proposal for the development of standardized nomenclature and terminology for chrysophycean statospores. In: Kristiansen, J. & Anderson, R.A. (eds.) Chrysophytes aspects and problems. Cambridge University Press, Cambridge, pp. 317–328.

Duff, K.E. & Smol, J.P. (1995) Chrysophycean cyst assemblages and their relationship to water chemistry in 71 Adirondack Park (New York, USA) lakes. Archiv für Hydrobiologie 134: 307–336.

de Jong, K., & Kamenik, C. (2011) Validation of chrysophyte stomatocysts-based cold-season climate reconstruction from high-Alpine Lake Silvaplana, Switzerland. Journal of Quaternary Science, 26: 268–275.

de Jong, K., Kamenik, C., Westover, K. & Grosjean, M. (2013) A chrysophyte stomatocyst-based reconstruction of cold-season air temperature from Alpine Lake Silvaplana (AD 1500–2003); methods and concepts for quantitative inferences. Journal of Paleolimnology 50: 519–533.

Duff, K.E. & Smol, J.P. (1991) Morphological descriptions and stratigraphic distributions of the chrysophycean stomatocysts from a recently acidified lake (Adirondack Park, N.Y.). Journal of Paleolimnology 5: 73–113.

Duff, K.E., Zeeb, B.A. & Smol, J.P. (1995) Atlas of Chrysophycean Cysts. Kluwer Academic Press, Dordrecht, Netherlands, 189 pp.

Ehrenberg, C.G. (1832) Über die Entwickelung und Lebensdauer der Infusionsthiere; nebst ferneren Beiträgen zu einer Vergleichung ihrer organischen Systeme. Abhandlungen der Königlichen Akademie Wissenschaften zu Berlin, Physikalische Klasse 1831: 1–154, pls I–IV.

Ehrenberg, C.G. (1843) Mittheilungen über 2 neue asiatische Lager fossiler Infusorien-Erden aus dem russischen Trans-Kaukasien (Grusien) und Sibirien. Bericht über die zur Bekanntmachung geeigneten Verhandlungen der Königlich-Preussischen Akademie der Wissenschaften zu Berlin 1843: 43–49.

Ehrenberg, C.G. (1843) Verbreitung und Einfluss des mikroskopischen Lebens in Süd-und Nord-Amerika. Abhandlungen der Königlichen Akademie der Wissenschaften zu Berlin 1841: 291–466, pls 1–4.

English, J. & Potapova, M. (2009) Aulacoseira pardata sp. nov., A. nivalis comb. nov., A. nivaloides comb. et stat. nov., and their Occurrences in Western North America. Proceedings of the Academy of Natural Sciences of Philadelphia 158: 37–48.

Facher, E. & Schmidt, R. (1996) A siliceous chrysophycean cyst˗based pH transfer function for Central European lakes. Journal of Paleolimnology 16: 275–321.

Firsova, A.D., Vorobyova, S.S. & Likhoshway, Y.V. (2012) Chrysophycean Stomatocysts in the Upper Pleistocene and Holocene Sediments from Lake Hovsgol, Northern Mongolia. International Journal of Geosciences 3: 664–674.

Fleitmann, D., Mudelsee, M., Burns, S.J., Bradley, R.S., Kramers, J. & Matter, A. (2008) Evidence for a widespread 18 climatic anomaly at around 9.2 ka before present. Paleoceanography 23: PA1102.

Grunow, A. (1882) Beiträge zur Kenntnis der fossilen Diatomeen Österreich-Ungarns. In: Mojsisorics, E. & Neumayer, N. (eds), Beiträge zur Paläontologie Österreich-Ungarns und des Orients 2: 136–159.

Huber, K., Kamenik, C., Weckström, K. & Schmidt, R. (2009) Taxonomy, stratigraphy, and palaeoecology of chrysophyte cysts from a Late Glacial sediment core section of Längsee, Austria. Nova Hedwigia 89: 245–261.

Hustedt, F. (1943) Die Diatomeenflora einiger Hochgebirgseen der Landschaft Davos in den Schweizer Alpen. Archiv für Hydrobiology 43: 124–197.

Jancsik, P. (2007) A Retyezát˗hegység (The Retezat Mountains). Pallas˗Akadémia Könyvkiadó, Csíkszereda, 140 pp.

Kamenik, C. (2010) Stom@ocysts & Co—web applications to bring the research community together via the Internet. Nova Hedwigia 136: 311–323.

Kamenik, C., & Schmidt, R. (2005a) Chrysophyte resting stages: a tool for reconstructing winter/spring climate from Alpine lake sediments. Boreas 34: 477–489.

Kamenik, C. & Schmidt, R. (2005b) Computer-aided SEM analysis of chrysophyte stomatocysts. Nova Hedwigia Beiheft 128: 269–274.

Kamenik, C., Schmidt, R., Koinig, K.A., Agusti-Panareda, A., Thompson, R. & Psenner, R. (2001) The chrysophyte stomatocyst composition in high alpine lake (Gossenköllesee, Tyrol) in relation to seasonality, temperature and land-use. Nova Hedwigia, Beiheft 122: 1–22.

Korponai, J., Magyari, E.K., Buczkó, K., Iepure, S., Namiotko, T., Czakó, D., Kövér, Cs. & Braun, M. (2011) Cladocera response to Late Glacial to Early Holocene climate change in a South Carpathian mountain lake. Hydrobiologia 676: 223–235.

Krammer, K. (1991) Morphology and taxonomy of some taxa in the genus Aulacoseira Thwaites (Bacillariophyceae). I. Aulacoseira distans and similar taxa. Nova Hedwigia 52: 89–112, pls I–X.

Krammer, K. (1997) Die cymbelloiden Diatomeen. Eine Monographie der welweit bekannten Taxa. Teil 1. Allgemeines und Encyonema Part. Bibliotheca Diatomologica 36: 1–382.

Kullenberg, B. (1947) The piston core-sampler. Svenska Hydrografisk-Biologiska Kommissionens Skrifter 1: 1–46.

Kützing, F.T. (1833) Synopsis diatomearum oder Versuch einer systematischen Zusammenstellung der Diatomeen. Linnaea 8: 529–620, pls XIII–XIX.

Kützing, F.T. (1844) Die Kieselschaligen Bacillarien oder Diatomeen. pp. 152, pls 1–30. Nordhausen.

Lange-Bertalot, H. & Genkal, S.I. (1999) Diatomeen aus Siberien. I. Insel im Arktischen Ozean (Yugorsky-Shar Strait) [Diatoms from Siberia I - Islands in the Arctic Ocean (Yugorsky-Shar Strait)]. In: (Lange–Bertalot, H. ed), Iconographia Diatomologica Königstein: Koeltz Scientific Books. 6, 303 pp.

Lange-Bertalot, H. & Krammer, K. (1989) Achnanthes eine Monographie der Gattung mit Definition der Gattung Cocconeis and Nachtragen zu den Naviculaceae. Bibliotheca Diatomologica, Cramer, Berlin–Stuttgart, 18, 393 pp.

Lotter, A.F., Appleby, P.G., Bindler, R., Dearing, J.A., Grytnes, J.A., Hofmann, W., Kamenik, C., Lami, A., Livingstone, D.M., Ohlendorf, C., Rose, N. & Sturm, M. (2002) The sediment record of the past 200 years in a Swiss high˗alpine lake: Hagelseewli (2339 m a.s.l.). Journal of Paleolimnology 28: 111–127.

Lotter, A.F., Birks, H.J.B., Hofmann, W. & Marchetto, A. (1997) Modern diatom, cladocera, chironomid, and chrysophyte cyst assemblages as quantitative indicators for the reconstruction of past environmental conditions in the Alps. I. Climate. Journal of Paleolimnology 18: 395–420.

Lowe, J.J., Rasmussen, S.O., Björck, S., Hoek, W.Z., Steffensen, J.P., Walker, M.J.C., Yu, Z. C. & I. Grp. 2008. Synchronisation of palaeoenvironmental events in the North Atlantic region during the Last Termination: a revised protocol recommended by the INTIMATE group. Quaternary Science Reviews 27: 6–17.

Magyari, E.K., Braun, M., Buczkó, K., Hubay, K. & Bálint, M. (2009) Radiocarbon chronology of glacial lake sediments in the Retezat Mts (South Carpathians, Romania): a window to Late Glacial and Holocene climatic and palaeoenvironmental changes. Central European Geology 52: 225–248.

Magyari, E.K., Jakab, G., Bálint, M., Kern, Z., Buczkó, K. & Braun, M. (2012) Rapid vegetation response to lateglacial and early Holocene climatic fluctuation in the South Carpathian Mountains (Romania). Quaternary Science Reviews 35: 116–130.

Magyari, E.K., Demény, A., Buczkó, K., Kern, Z., Vennemann, T., Fórizs, I., Vincze, I., Braun, M., Kovács, J.I., Udvardi, B. & Veres, D. (2013) A 13,600-year diatom oxygen isotope record from the South Carpathians (Romania): Reflection of winter conditions and possible links with North Atlantic circulation changes. Quaternary International 293: 136–149.

Mereschkowsky, C. (1902) On Sellaphora, a new genus of diatoms. Annals and Magazine of Natural History, Series 7 9: 185–195.

Nicholls, K.H. (1981) Chrysococcus furcatus (Dolg.) comb. nov.: a new name for Chrysastrella furcata (Dolg.) Defl. based on the discovery of the vegetative stage. Phycologia 20: 16–21.

O’Meara, E. (1875) Report on the Irish Diatomaceae. Proceedings of the Royal Irish Academy, Series 2 2: 235–425, pls 26–34.

Østrup, E. (1910) Danske Diatoméer. Kjøbenhavn, Copenhagen, C.A. Reitzels Boghandel. pp. 1–323, 5 pls.

Péterfi, L.S. (1967) Studies on the Rumanian Chrysophyceae. I. Nova Hedwigia 13: 117–137.

Pla, S. (2001) Chrysophycean cysts from the Pyrenees. Bibliotheca Phycologica, Cramer, Berlin, Germany, 109, 179 pp.

Pla, S. & Catalan, J. (2005) Chrysophyte cysts from lake sediments reveal the submillennial winter/spring climate variability in the northwestern Mediterranean region throughout the Holocene. Climate Dynamics 24: 263–278.

Pla, S., Camarero, L. & Catalan, J. (2003) Chrysophyte cyst relationships to water chemistry in Pyrenean lakes (NE Spain) and their potential for environmental reconstruction. Journal of Paleolimnology 30: 21–34.

Pla, S. & Anderson, N.J. (2005) Environmental factors correlated with chrysophyte cyst assemblages in low arctic lakes of south-west Greenland. Journal of Phycology 41: 957–974.

Pla˗Rabes, S. & Catalan, J. (2011) Deciphering chrysophyte responses to climate seasonality. Journal of Paleolimnology 46: 139–150.

Preisig, H.R. (1995) A modern concept of chrysophyte classification. In Sandgren, C.D., Smol, J.E. & Kristiansen, J. (eds.) Chrysophyte Algae: Ecology, Phylogeny and Development. Cambridge University Press, Cambridge, pp. 46–74.

Round, F.E. & Bukhtiyarova, L. (1996) Four new genera based on Achnanthes (Achnanthidium) together with a re-definition of Achnanthidium. Diatom Research 11: 345–361.

Schmidt, R., Kamenik, C., Roth, M. (2007) Siliceous algae-based seasonal temperature inferences and indicator pollen tracking 4,000 years of climate/land-use interactions in the southern Austrian Alps. Journal of Paleolimnology 38: 541–554.

Simonsen, R. (1979) The diatom system: ideas on phylogeny. Bacillaria 2: 9–71.

Smith, W. (1853) A synopsis of the British Diatomaceae; with remarks on their structure, function and distribution; and instructions for collecting and preserving specimens. 1 pp, 89 pp., 31 pls. London: John van Voorst.

Soróczki˗Pintér, É., Buczkó, K., Braun, M. & Magyari, E.K. (2012) Későglaciális és holocén vízszintváltozások a Retyezátban egy gleccsertó kovaalga összetétele alapján. (Late glacial and Holocene diatom based lake level reconstruction in a glacial lake in Retezat Mountains (Romania)). Hidrológiai Közlöny 92: 64–67.

Soróczki-Pintér, É., Magyari, E.K. & Buczkó, K. (2013) Preuve fondée sur les algues siliceuses de l’augmentation du niveau d’eau et du refroidissement à court terme autour de 9.2-ka dans les Carpates du Sud, Roumanie. (Siliceous algae based evidence for short-term lake level increase and cooling around 9.2-ka BP in the South Carpathian Mountains, Romania). In.: Rimet, F., Bouchez, A., Ector, L. & Montuelle, B. (eds.) Livre des résumés et programme. 7th CE-Diatom Meeting, 32nd ADLaF Meeting. Thonon-les-Bains, France, 16–20 sept. 2013, 77–80. pp

Stewart, K., Gregory-Eaves, I., Zeeb, B.A. & Smol, J.P. (2000) Covariation among Alaskan chrysophyte stomatocyst assemblages and environmental gradients: A comparison with diatom assemblages. Nordic Journal of Botany 20: 357–368.

Tóth, M., Magyari, E.K., Brooks, S.J., Braun, M., Buczkó, K., Bálint, M. & Heiri, O. (2012) Lateglacial summer temperatures in the Southern Carpathians (Romania): a chironomid-based reconstruction. Quaternary Research 77: 122–131.

Thwaites, G.H.K. (1848) Further observations on the Diatomaceae with descriptions of new genera and species. Annals and Magazine of Natural History, Series 2 1: 161–172, pls XI, XII.

Van de Vijver, B. &. Beyens, L. (1997a) The Chrysophyte Stomatocyst Flora of the Moss Vegetation from Strømness Bay Area, South Georgia. Archiv für Protistenkunde 148: 505–520.

Van de Vijver, B. & Beyens, L. (1997b) The Subfossil Chrysophyte Cyst Flora of Some Peat Samples from Kerguelen Islands. Archiv für Protistenkunde 148: 491–503.

Vorobyova, S.S., Pomazkina, G.V., Baranova, E.Yu., Likhoshway, Ye.V. & Sandgren, C.D. (1996) Chrysophycean cysts (stomatocysts) from Lake Baikal and Irkutsk Reservoir, Siberia. Journal of Paleolimnology 15: 271–277.

Wilkinson, A.N., Zeeb, B.A., Smol, J.P., & Douglas, M.S.V. (1997) Chrysophyte stomatocyst assemblages associated with periphytic, high Arctic pond environments. Nordic Journal of Botany 17: 95–112.

Wilkinson, A.N. & Smol, J.P. (1998) Chrysophycean stomatocyst flora from south-central Ontario lakes. Canadian Journal of Botany 76: 836–862.

Wilkinson, A.N., Zeeb, B. & Smol, J.P. (2001) Atlas of Chrysophycean Cysts, Volume II. Kluwer Academic Publishers, Dordrecht, 180 pp.

Williams, D.M. & Round, F.E. (1987) Revision of the genus Fragilaria. Diatom Research 2: 267–288.

Zeeb, B.A. & Smol, J.P. (2001) Chrysophyte scales and cysts. In: Smol, J.P., Birks, H.J.B. & Last, W.M. (eds.) Tracking Environmental Change Using Lake Sediments Volume 3: Terrestrial, Algal, and Siliceous Indicators. Kluwer Academic Publishers, Dordrecht, pp. 203–223.


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