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DOI: http://dx.doi.org/10.11646/phytotaxa.277.1.1

Multilocus phylogeny reveals Gibsmithia hawaiiensis (Dumontiaceae, Rhodophyta) to be a species complex from the Indo-Pacific, with the proposal of G. eilatensis sp. nov.

DANIELA GABRIEL, STEFANO G.A. DRAISMA, THOMAS SAUVAGE, WILLIAM E. SCHMIDT, TOM SCHILS, PHAIK-EEM LIM, D. JAMES HARRIS, SUZANNE FREDERICQ

Abstract


Gibsmithia hawaiiensis is a peculiar red alga characterized by furry gelatinous lobes arising from a cartilaginous stalk. The species has been recorded from tropical reef systems throughout the Indo-Pacific. A multilocus phylogeny (UPA, rbcL, COI-5P) of 36 specimens collected throughout the species distribution range, showed high genetic diversity at species level. Two major groups were identified, each consisting of multiple lineages. Genetic variability was low in the Hawaiian Islands and the northern Red Sea and high in the Western Indian Ocean and the Coral Triangle, where lineages overlap in distribution. Genetic distances suggest that G. hawaiiensis represents a complex of five cryptic species, with no difference observed in the external morphology corresponding to separate lineages. Anatomical and reproductive differences were observed at the microscopic level for the lineage from the Red Sea, which is here described as G. eilatensis sp. nov. The geographic range of the species complex is here expanded to include Madagascar, the Red Sea and the Indo-Malay region, and the generitype seems endemic to the Hawaiian Islands. Algal diversity on coral reef systems is discussed from a conservation perspective using G. hawaiiensis as an example.


Keywords


Cryptic species, DNA barcoding, Dudresnaya, Gigartinales, systematics, Algae, Indo-Pacific

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References


Abbott, I. (1999) Marine red algae of the Hawaiian Islands. Bishop Museum Press, Honolulu, Hawaii, 477 pp.

Atmadja, W.S. & Prud’homme van Reine, W.F. (2010) Checklist of the Seaweed Species Biodiversity of Indonesia. Coral Reef Information and Training Centre, Jakarta, 72 pp.

Bickford, D., Lohman, D.J., Sodhi, N.S., Ng, P.K.L., Meier, R., Winker, K., Ingram, K.K. & Das, I. (2007) Cryptic species as a window on diversity and conservation. Trends in Ecology and Evolution 22: 148–155.

http://dx.doi.org/10.1016/j.tree.2006.11.004

Brodie, J., Andersen, R.A., Kawachi, M. & Millar, A.J.K. (2009) Endangered algal species and how to protect them. Phycologia 48: 423–438.

http://dx.doi.org/10.2216/09-21.1

Clarkston, B.E. & Saunders, G.W. (2012) An examination of the red algal genus Pugetia (Kallymeniaceae, Gigartinales), with descriptions of Salishia firma gen. & comb. nov., Pugetia cryptica sp. nov. and Beringia wynnei sp. nov. Phycologia 51: 33–61.

http://dx.doi.org/10.2216/11-01.1

Clarkston, B.E. & Saunders, G.W. (2013) Resolving species diversity in the red algal genus Callophyllis (Kallymeniaceae, Gigartinales) in Canada using molecular assisted alpha taxonomy. European Journal of Phycology 48: 27–46.

http://dx.doi.org/10.1080/09670262.2013.767943

Crouan, P.L. & Crouan, H.M. (1835) Observations microscopique sur le genre Mesogloia Agardh. Annales des Sciences Naturelles, Botanique 9: 98–100.

De Clerck, O., Guiry, M.D., Leliaert, F., Samyn, Y. & Verbruggen, H. (2013) Algal taxonomy: a road to nowhere? Journal of Phycology 49: 215–225.

http://dx.doi.org/10.1111/jpy.12020

Doty, M.S. (1963) Gibsmithia hawaiiensis gen. n. et sp. n. Pacific Science 17: 458–465.

Draisma, S.G.A. (2012) Macroalgae. In: Kassem, K., Hoeksema, B. & Y.A. Affendi (Eds.) Semporna Marine Ecological Expedition. Kota Kinabalu Malaysia, pp. 77–117.

Freshwater, D.W., Tudor, K., O’Shaughnessy, K. & Wysor, B. (2010) DNA barcoding in the red algal order Gelidiales: Comparison of COI with rbcL and verification of the “barcoding gap”. Cryptogamie, Algologie 31: 435–449.

Gabriel, D., Schils, T., Neto, A.I., Paramio, L. & Fredericq, S. (2009) Predaea feldmannii subsp. azorica (Nemastomataceae, Nemastomatales), a new subspecies of red algae (Rhodophyta) from the Azores. Cryptogamie, Algologie 30: 251–271.

Guiry, M.D. & Guiry, G.M. (2016) AlgaeBase. World-wide electronic publication. National University of Ireland, Galway (accessed 20 May 2016)

Hughey, J.R., Silva, P.C. & Hommersand, M.H. (2001) Solving taxonomic and nomenclatural problems in Pacific Gigartinaceae (Rhodophyta) using DNA from type material. Journal of Phycology 37: 1091–1109.

http://dx.doi.org/10.1046/j.1529-8817.2001.01048.x

Huisman, J.M., Abbott, I.A. & Smith, C.M. (2007) Hawaiian reef plants. University of Hawaii Sea Grant College Program, Honolulu, Hawaii, 264 pp.

Karam-Kerimian, T.B. (1976) Structure, reproduction et discussion sur la position systématique du genre Gibsmithia (Rhodophyceae). Bulletin du Muséum National D’Historie Naturelle, Série 3 365: 21–32.

Kraft, G. (1986) The genus Gibsmithia (Dumontiaceae, Rhodophyta) in Australia. Phycologia 25: 423–447.

http://dx.doi.org/10.2216/i0031-8884-25-4-423.1

Kylin, H. (1925) The marine red algae in the vicinity of of the Biological Station at Friday Harbor, Washington. Lunds Universitets Årsskrift, Ny Följd, Andra Afdelningen 21: 1–87.

Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) PartitionFinder: Combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution 29: 1695–1701.

http://dx.doi.org/10.1093/molbev/mss020

Le Gall, L. & Saunders, G.W. (2010) DNA barcoding is a powerful tool to uncover algal diversity: A case study of the Phyllophoraceae (Gigartinales, Rhodophyta) in the Canadian Flora. Journal of Phycology 46: 374–389.

http://dx.doi.org/10.1111/j.1529-8817.2010.00807.x

Le Jolis, A. (1863) Liste des algues marines de Cherbourg. Mémoires de la Société Impériale des Sciences Naturelles de Cherbourg 10: 5–168.

Lee, R.K.S. (1963) The structure and reproduction of Dudresnaya hawaiiensis sp. nov. (Rhodophyta). American Journal of Botany 50: 315–319.

Lin, S., Fredericq, S. & Hommersand, M. (2001) Systematics of the Delesseriaceae (Ceramiales, Rhodophyta) based on large subunit rDNA and rbcL sequences, including the Phycodryoideae, subfam. nov. Journal of Phycology 37: 881–899.

http://dx.doi.org/10.1046/j.1529-8817.2001.01012.x

Maddison, W.P. & Maddison, D.R. (2005) MacClade 4: analysis of phylogeny and character evolution. Version 4.8a. Available from: http://macclade.org (accessed 1 September 2016)

Millar, A. & Kraft, G. (1984) The red algal genus Acrosymphyton (Dumontiaceae , Cryptonemiales) in Australia. Phycologia 23: 135–145.

http://dx.doi.org/10.2216/i0031-8884-23-2-135.1

N’Yeurt, A.D.R. & Payri, C.E. (2010) Marine algal flora of French Polynesia III. Rhodophyta, with additions to the Phaeophyceae and Chlorophyta. Cryptogamie. Algologie 31: 3–196.

Okamura, K. (1902) Nippon Sorui-meii. Keigyosha, Tokyo, 276 pp.

Payo, D.A., Leliaert, F., Verbruggen, H., D’hondt, S., Calumpong, H.P. & De Clerck, O. (2013) Extensive cryptic species diversity and fine-scale endemism in the marine red alga Portieria in the Philippines. Proceedings of the Royal Society B: Biological Sciences 280: 2012.2660.

http://dx.doi.org/10.1098/rspb.2012.2660

Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.

http://dx.doi.org/10.1093/bioinformatics/btg180

Saunders, G.W. (2005) Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 360: 1879–88.

http://dx.doi.org/10.1098/rstb.2005.1719

Saunders, G.W. (2008) Barcode examination of the red algal family Dumontiaceae in Canadian waters reveals substantial cryptic species diversity. 1. The foliose Dilsea-Neodilsea complex. Botany 789: 773–789.

http://dx.doi.org/10.1139/B08-001

Schils, T. & Coppejans, E. (2002) Gelatinous red algae of the Arabian Sea, including Platoma heteromorphum sp. nov. (Gigartinales, Rhodophyta). Phycologia 41: 254–267.

http://dx.doi.org/10.2216/i0031-8884-41-3-254.1

Sherwood, A.R. & Presting, G.G. (2007) Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and cyanobacteria. Journal of Phycology 43: 605–608.

http://dx.doi.org/10.1111/j.1529-8817.2007.00341.x

Sherwood, A.R., Kurihara, A., Y Conklin, K., Sauvage, T. & Presting, G.G. (2010) The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings. BMC Plant Biology 10: 258.

http://dx.doi.org/10.1186/1471-2229-10-258

Stamatakis, A. (2008) The RAxML 7.0.4 Manual. Ludwig-Maximilians-Universität, Munich, 25 pp.

Tsuda, R.T. (2014) Endemism of marine algae in the Hawaiian Islands. Bishop Museum Occasional Papers 115: 23–27.

Womersley, H.B.S. (1994) The marine benthic flora of southern Australia - Part IIIA - Bangiophyceae and Florideophyceae (Acrochaetiales, Nemaliales, Gelidiales, Hildenbrandiales and Gigartinales sensu lato). Australian Biological Resources Study, Canberra, 508 pp.


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