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Allium pseudotelmatum (Amaryllidaceae), a new species of Allium sect. Codonoprasum from Croatia



Allium pseudotelmatum, a new species of A. sect. Codonoprasum, is described from the southern Dalmatia (Croatia). It is a late-summer flowering species with pentaploid (2n = 40) chromosome complement, occurring in synanthropic habitats (Citrus orchards with nutrient rich and fresh moist soil). Karyology, leaf anatomy, morphology, ecology, molecular (ITS) and taxonomic relationships with related species are examined for the species. It shows morphological relationships with the northern Dalmatian species A. telmatum from which it well differentiates for many morphological characters including the presence of aerial bulbils within the inflorescence, phenology, chromosome number and ecology. Confusion of A. pseudotelmatum with some specimens of A. oleraceum due to the presence of bulbils within the inflorescence is discussed.


bulbils, genome size, ITS, karyology, leaf anatomy, taxonomy, Monocots

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Abrahamson, W.G. (1980) Demography and vegetative reproduction. In: Solbrig, O.T. (Ed.) Demography and Evolution in Plant Populations. Blackwell, Oxford, pp. 89–106.

Aedo, C. (2013) Allium L. In: Castroviejo, S. (Ed.) Flora Iberica. Vol. XX. C.S.I.C., Madrid, pp. 220–273.

Åström, H. & Hæggström, C.A. (2004) Generative reproduction in Allium oleraceum (Alliaceae). Annales Botanici Fennici 40: 1–14.

Balao, F., Herrera, J. & Talavera, S. (2011) Phenotypic consequences of polyploidy and genome size at the microevolutionary scale: A multivariate morphological approach. New Phytologist 192: 256–265.

Beaulieu, J.M., Leitch, I.J., Patel, S., Pendharkar, A. & Knight, C.A. (2008) Genome size is a strong predictor of cell size and stomatal density in angiosperms. New Phytologist 179: 975–986.

Biel, B., Tan, K. & Tzanoudakis, D. (2006) A new autumn-flowering species of Allium (Liliaceae) from the island of Sifnos (Cyclades, Greece). Willdenowia 36 (Special Issue): 367–372.

Blanco, F.M. (1837) Flora de Filipinas: segun el sistema sexual de Linneo. C. Lopez, Manila, 387 pp.

Bogdanović, S., Brullo, S., Mitić, B. & Salmeri, C. (2008) A new species of Allium (Alliaceae) from Dalmatia, Croatia. Botanical Journal of the Linnean Society 158: 106–114.

Bogdanović, S., Brullo, S., Del Galdo, G.G. & Salmeri, C. (2009) A new autumn-flowering species of Allium (Alliaceae) from Croatia. Folia Geobotanica 44: 83–93.

Bogdanović, S., Brullo, C., Brullo, S., Del Galdo G., Musarella, C.M. & Salmeri C. (2011) Allium achaium Boiss. (Alliaceae), a critical species of Greek flora. Candollea 66: 57–64.

Brullo, C., Brullo, S., Fragman-Sapir, O., Del Galdo, G.G. & Salmeri, C. (2014) Allium therinanthum (Amaryllidaceae), a new species from Israel. Phytotaxa 164: 29–40.

Brullo, S. (2009) Criteria and techniques in the taxonomic investigation of the Mediterranean flora. Bocconea 23: 7–18.

Brullo, S., Pavone, P., Salmeri, C. & Scrugli, A. (1994) Cytotaxonomical notes on Allium savii Parl. (Alliaceae), a misappreciated Tyrrhenian element. Candollea 49: 271–279.

Brullo, S., Guglielmo, A., Pavone, P., Scelsi, F. & Terrasi M.C. (1996) Cytotaxonomic consideration of Allium fuscum Waldst. et Kit. (Liliaceae), a critical species of the European flora. Folia Geobotanica & Phytotaxonomica 31: 465–472.

Brullo, S., Pavone, P. & Salmeri, C. (1997a) Specie a fioritura tardiva del gruppo di Allium paniculatum nell′area mediterranea. Informatore Botanico Italiano 29: 300–302.

Brullo, S., Pavone, P. & Salmeri, C. (1997b) Allium oporinanthum (Alliaceae), a new species from the NW Mediterranean area. Anales del Jardín Botánico de Madrid 55: 297–302.

Brullo, S., Pavone, P. & Salmeri, C. (1997c) Allium anzalonei, eine neue Art für die italienische Flora. Sendtnera 4: 33–39.

Brullo, S., Guglielmo, A., Pavone, P. & Terrasi, M.C. (1998) Notes on Allium rhodopeum (Alliaceae), a neglected species from the E Mediterranean area. Plant Biosystems 132: 63–69.

Brullo, S., Pavone, P. & Salmeri, C. (1999) Allium archeotrichon (Alliaceae), a new species from Rhodos (Dodekannisos, Greece). Nordic Journal of Botany 19: 41–46.

Brullo, S., Guglielmo, A., Pavone, P. & Salmeri, C. (2001) Osservazioni tassonomiche e cariologiche sulle specie del ciclo di Allium paniculatum L. in Italia. Informatore Botanico Italiano 33: 500–506.

Brullo, S., Guglielmo, A., Pavone, P. & Salmeri, C. (2003a) Cytotaxonomical remarks on Allium pallens L. and its relationships with Allium convallarioides Grossh. Bocconea 16: 557–571.

Brullo, S., Guglielmo, A., Pavone, P., Salmeri, C. & Terrasi, M.C. (2003b) Three new species of Allium sect. Codonoprasum from Greece. Plant Biosystems 137: 131–140.

Brullo, S., Guglielmo, A., Pavone, P. & Salmeri, C. (2007) Cytotaxonomic considerations on Allium stamineum Boiss. group (Alliaceae). Bocconea 21: 325–343.

Brullo, S., Guglielmo, A., Pavone, P. & Salmeri, C. (2008) Taxonomical remarks on Allium dentiferum and its relationships with allied species. Taxon 57: 243–253.

Burešová, V. (2012) Comparative morphology of cytotypes of Allium oleraceum. MSc thesis. Olomouc, Palacky University, 67 pp.

Choi, H.J. & Oh, B.U. (2011) Partial revision of Allium (Amaryllidaceae) in Korea and northeastern China. Botanical Journal of the Linnean Society 167: 153–211.

Česchmedziev, I. (1979) Kariosistematicno izsledvane na vidove ot rod AlIium L. v Balgarija. Fitologija 11: 40–46.

Česchmedziev, I. (1992) Allium melanantherum complex in Bulgaria. In: Hanelt, P., Hammer, K. & Knüpffer, H. (Eds.) The genus Allium: taxonomic problems and genetic resources. Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, pp. 21–26.

Doležel, J. & Bartoš, J. (2005) Plant DNA flow cytometry and estimation of nuclear genome size. Annals of Botany 95: 99–110.

Doležel, J., Greilhuber, J., Lucretti, S., Meister, A., Lysák, M.A., Nardi, L. & Obermayer, R. (1998) Plant genome size estimation by flow cytometry: inter-laboratory comparison. Annals of Botany 82 (Suppl. A): 17–26.

Doležel, J., Greilhuber, J. & Suda, J. (2007) Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols 2: 2233–2244.

Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.

Duchoslav, M. (2001) Allium oleraceum and Allium vineale in the Czech Republic: distribution and habitat differentiation. Preslia 73: 173–184.

Duchoslav, M. (2009) Effects of contrasting habitats on the phenology, seasonal growth, and dry-mass allocation pattern of two bulbous geophytes (Alliaceae) with partly different geographic ranges. Polish Journal of Ecology 57: 15–32.

Duchoslav, M. , Šafářová, L. & Krahulec, F. (2010) Complex distribution patterns, ecology and coexistence of ploidy levels of Allium oleraceum (Alliaceae) in the Czech Republic. Annals of Botany 105: 719–735.

Duchoslav, M., Šafářová, L. & Jandová, M. (2013) Role of adaptive and non-adaptive mechanisms forming complex patterns of genome size variation in six cytotypes of polyploid Allium oleraceum (Amaryllidaceae) on a continental scale. Annals of Botany 111: 419–431.

Fazekas, A.J., Kuzmina, M.L., Newmaster, S.G. & Hollingsworth, P.M. (2012) DNA barcoding methods for land plants. Methods in Molecular Biology 858: 223–852.

Fialová, M. & Duchoslav, M. (2014) Response to competition of bulbous geophyte Allium oleraceum differing in ploidy level. Plant Biology 16: 186–196.

Fialová, M., Jandová, M., Ohryzek, J. & Duchoslav, M. (2014) Biology of the polyploid geophyte Allium oleraceum (Amaryllidaceae): variation in size, sexual and asexual reproduction and germination within and between tetra-, penta- and hexaploid cytotypes. Flora 209: 312–324.

Fialová, R. (1996) Polyploid complexes in the genus Allium. PhD thesis, Olomouc, Palacký University, 127 pp.

Fraga, P. (2002) Notes i contribucion al coneixement de la flora de Menorca (IV). El grup d′Allium paniculatum L. (sect. Codonoprasum Reichenb.) a Menorca. Bolletí de la Societat d’Història Natural de les Balears 45: 93–104.

Friesen, N., Fritsch, R.M. & Blattner, F.R. (2006) Phylogeny and new intrageneric classification of Allium (Alliaceae) based on nuclear ribosomal DNA ITS sequences. Aliso 22: 372–375.

Garbari, F., Miceli, P. & Raimondo, F.M. (1994) Allium lehmanii subsp. castellanense (Alliaceae) nuova entita dell’interno siciliano. Atti della Società Toscana de Scienze Naturali di Pisa serie B 101: 137–146.

Greilhuber, J., Doležel, J., Lysák, M.A. & Bennett, M.D. (2005) The origin, evolution and proposed stabilization of the terms ‘Genome Size’ and ‘C-Value’ to describe nuclear DNA contents. Annals of Botany 95: 255–260.

Hadžiablahović, S. (2005) Contribution to the flora of Montenegro and FR Yugoslavia. Hacquetia 4: 103–110.

Hæggström, C.A. & Åström, H. (2005) Allium oleraceum (Alliaceae) in Finland: distribution, habitats and accompanying vascular plant species. Memoranda Societatis pro Fauna et Flora Fennica 81: 1–18.

Hanelt, P. (1996) Taxonomic problems in Mediterranean Allium, and relationships with non-Mediterranean Allium groups. Bocconea 5: 259–265.

Hanelt, P., Schultze-Motel, J., Fritsch, R., Kruse, J., Maass, H., Ohle, H. & Pistrick, K. (1992) Infrageneric grouping of Allium. The Gatersleben approach. In: Hanelt, P., Hammer, K. & Knüpffer, H. (Eds.) The genus Allium: taxonomic problems and genetic resources. Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, pp. 107–123.

Hultén, E. & Fries, M. (1986) Atlas of north European vascular plants north of the Tropic of Cancer I. Köningstein, Koeltz, 1172 pp.

IUCN (2012) IUCN Red List Categories and Criteria: Version 3.1. Second edition. IUCN, Gland, Switzerland, 32 pp.

Jauzein, P. & Tison, J.M. (1999) Hypothéses sur les liens entre Allium oleraceum L., Allium oporinanthum Brullo, Pavone & Salmeri et Allium savii Parl. Journal de Botanique de la Société Botanique de France 11: 55–58.

Jauzein, P. & Tison, J.M. (2001) Etude analytique du genre Allium L., sous-genre Codonoprasum (Reichenb.) Zahar., section Codonoprasum Reichenb., en France. Journal de Botanique de la Société Botanique de France 15: 29–50.

Jauzein, P., Tison, J.M., Deschâtres, R. & Couderc, H. (2002) Allium corsicum Jauzein, J.M. Tison, Deschâtres & H. Couderc spec. nova, espèce méconnue de la flore corse. Candollea 56: 301–314.

Kalpoutzakis, E., Trigas, P. & Constantinidis, T. (2012) Allium orestis sp. nov. (Amaryllidaceae) from Parnon and Taigetos mountains, south Peloponnisos, Greece. Nordic Journal of Botany 30: 195–200.

Karpavičienė, B. (2012) Morphological, reproductive and karyological variability in Allium oleraceum in Lithuania. Biologia 67: 278–283.

Karpavičienė, B. (2017) Causes of variation in sexual and asexual reproduction in diploid and triploid populations of Allium scorodoprasum. Plant Systematics and Evolution 303: 105–115.

Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Mentjies, P. & Drummond, A. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28: 1647–1649.

Kim, S., Rayburn, A.L., Boe, A. & Lee, D.K. (2012) Neopolyploidy in Spartina pectinata Link: 1. Morphological analysis of tetraploid and hexaploid plants in a mixed natural population. Plant Systematics and Evolution 298: 1073–1083.

Koçyiğit, M. & Özhatay, N. (2012) Allium maraschicum sp. nov. (Alliaceae) from Turkey. Nordic Journal of Botany 30: 553–559.

Koçyiğit, M., Yeşil, Y. & Koyuncu, M. (2016) Allium dumanii (A. sect. Codonoprasum, Amaryllidaceae), a new species from E Turkey. Willdenowia 46: 113–119.

Kollmann, F., Schmida, A. & Cohen, O. (1991) Allium tardiflorum Kollmann & Schmida, a new autumn flowering species. Herbertia 46: 23–32.

Krahulec, F. (1980) Epidermal characters of Allium species autochthonous in Czechoslovakia: their pattern, taxonomic and ecological relationships. Preslia 52: 299–309.

Levan, A. (1937) Cytological studies in Allium paniculatum group. Hereditas 23: 317–370.

Levan, A., Freda, K. & Sandberg, A.A. (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.

Li, Q.Q., Zhou, S.D., He, X.J., Yu, Y., Zhang, Y.C. & Wei, X.Q. (2010) Phylogeny and biogeography of Allium (Amaryllidaceae: Allieae) based on nuclear ribosomal internal transcribed spacer and chloroplast rps16 sequences, focusing on the inclusion of species endemic to China. Annals of Botany 106: 709–733.

Li, Q.Q., Zhou, S.D., Huang, D.Q., He, X.J. & Wei, X.Q. (2016) Molecular phylogeny, divergence time estimates and historical biogeography within one of the world’s largest monocot genera. AoB Plants 8: plw041.

Lillie, R.D. (1951) Simplification of the manufacture of Schiff reagent for use in histochemical procedures. Stain Technology 25: 163–165.

Lin, C-Y. & Tan, D-Y. (2015) The taxonomic significance of leaf epidermal micromorphological characters in distinguishing 43 species of Allium (Amaryllidaceae) from Central Asia. Pakistan Journal of Botany 47: 1979–1988.

Linnaeus, C. (1753) Species Plantarum, 1. Laurentii Salvii, Holmiae, 560 pp.

Linnaeus, C. (1759) Systema Naturae. 10.2. Laurentii Salvii, Holmiae, 824 pp.

Linnaeus, C. (1762) Species Plantarum, ed. 2, vol. 1. Laurentii Salvii, Holmiae, 784 pp.

Milović, M. (2015) A new contribution to the knowledge of the vascular flora of the Krka National Park (North Dalmatia, Croatia). Glasnik Hrvatskog Botaničkog Društva 4: 22–29.

Mirzaghaderi, G. & Marzangi, K. (2015) IdeoKar: an ideogram constructing and karyotype analysing software. Caryologia 68: 31–35.

Mössler, J.C. (1827) Gemeinnütziges Handbuch der Gewächskunde, ed. 2, 1. J.F. Hammerich, Altona, 784 pp.

Nikolić, T. (Ed.) (2016) Flora Croatica Database (FDC). University of Zagreb, Department of Botany, Zagreb. Available from: (accessed 1 November 2016)

Pančić, J. (1883) Građa za floru kneževine Bugarske [Elementa ad floram principatus Bulgariae]. Glasnik Srpskog Učenog Društva 53: 161–231.

Parlatore, F. (1857) Flora italiana 2. Le Monnier, Firenze, 638 pp.

Pastor, J. (1982) Karyology of Allium species from the Iberian Peninsula. Phyton (Horn) 22: 171–200.

Ramsey, J. & Schemske, D.W. (1998) Pathways, mechanisms, and rates of polyploid formation in flowering plants. Annual Review of Ecology and Systematics 29: 467–501.

Rechinger, K.H. (1961) Die Flora von Euboea. Botanische Jahrbücher für Systematik, Pflanzengeschichte und Pflanzengeographie 80: 294–465.

Rey, C., Rey, S., Kaplan, K. & Bovio, M. (2015) Allium oporinanthum Brullo, Pavone & Salmeri, new species for the Aoste Valley and for Italy. Revue Valdôtaine D’histoire Naturelle 69: 21–40.

Šafářová, L. & Duchoslav, M. (2010) Cytotype distribution in mixed populations of polyploid Allium oleraceum measured at a microgeographic scale. Preslia 82: 107–126.

Salmeri, C., Brullo, C., Brullo, S., Del Galdo, G.G. & Moysiyenko, I.I. (2016) What is Allium paniculatum? Establishing taxonomic and molecular phylogenetic relationships within A. sect. Codonoprasum. Journal of Systematics and Evolution 54: 123–135.

Seregin, A.P., Anačkov, G. & Friesen, N. (2015) Molecular and morphological revision of the Allium saxatile group (Amaryllidaceae): geographical isolation as the driving force of underestimated speciation. Botanical Journal of the Linnean Society 178: 67–101.

Stearn, W.T. (1980) Allium L. In: Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H. & Walters, S.M. (Eds.) Flora Europaea 5. Cambridge University Press, Cambridge, pp. 49–69.

Stearn, W.T. (1981) The genus Allium in the Balkan Peninsula. Botanische Jahrbücher für Systematik, Pflanzengeschichte und Pflanzengeographie 102: 201–213.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 4.0. Molecular Biology and Evolution 30: 2725–2729.

Tatić, B. (1975) Allium L. In: Josifović, M. (Ed.) Flora SR Srbije 7. Srpska Akademija nauka i umetnosti, Beograd, pp. 568–592.

Tison, J.M., Jauzein, P. & Michaud, H. (2014) Flore de la France mediterraneene continentale. Naturalia Publications, 2080 pp.

Tzanoudakis, D. (2000) Allium aegilicum (Alliaceae), a new autumn flowering species from the island of Antikithira (Greece). Botanica Chronica (Patras) 13: 81–86.

Tzanoudakis, D. & Kypriotakis, Z. (1993) Allium platakisii, a new species of the Greek insular flora. Flora Meditteranea 3: 309–314.

Tzanoudakis, D. & Tan, K. (2000) Allium samothracicum Tzanoudakis, Strid & Kit Tan, a new species from the North Aegean area, Greece. Portugaliae Acta Biologica 19: 355–360.

Tzanoudakis, D. & Trigas, P. (2015) Allium occultum, a new species of A. sect. Codonoprasum (Amaryllidaceae) from Skiros Island (W Aegean, Greece). Phytotaxa 202: 135–142.

Waldstein, F. de P.A. von & Kitaibel, P. (1808) Descriptiones et icones plantarum rariorum Hungariae 3. Typis Matthiae Andreae Schmidt, Vienna, 312 pp.

Webb, P.B. & Berthelot, S. (1848) Phytografia canariensis. 3(2), sect. III. Béthune, Paris, pp. 233–479.

White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, pp. 315–322.

Yousaf, Y., Shinwari, Y.K., Asghar, R. & Parveen, A. (2008) Leaf epidermal anatomy of selected Allium species, family Alliaceae from Pakistan. Pakistan Journal of Botany 40: 77–90.

Zahariadi, C. (1975) Le sous-genre Codonoprasum (Genre Alium L., fam. Alliaceae Agardh 1858) en Grece et en Roumainie. IIe partie. Biologia Gallo-Helenica 6: 27–64.


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