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Morphological and molecular characterization of cyanobacterial isolates from the mouth of the Amazon River


 
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1. Title Title of document

Morphological and molecular characterization of cyanobacterial isolates from the mouth of the Amazon River

 
2. Creator Author's name, affiliation, country ELANE D. CUNHA DE OLIVEIRA; IEPA, Institute of Scientific and Technological Research of the State of Amapá, 68.903-419, Macapá-AP Brazil. Bionorte Post-Graduate Program, UNIFAP, Federal University of Amapá, 68903-419, Macapá-AP, Brazil.; Brazil
 
2. Creator Author's name, affiliation, country ALAN C. DA CUNHA; Bionorte Post-Graduate Program, UNIFAP, Federal University of Amapá, 68903-419, Macapá-AP, Brazil.; Brazil
 
2. Creator Author's name, affiliation, country NATALINA B. DA SILVA; Scientific Initiation student at IEPA, Institute of Scientific and Technological Research of the State of Amapá, 68.903-419, Macapá-AP Brazil.; Brazil
 
2. Creator Author's name, affiliation, country RAQUEL CASTELO-BRANCO; CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, 4050-208 Matosinhos, Portugal.; Portugal
 
2. Creator Author's name, affiliation, country JOÃO MORAIS; CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, 4050-208 Matosinhos, Portugal.; Portugal
 
2. Creator Author's name, affiliation, country MARIA PAULA C. SCHNEIDER; UFPA, Federal University of Pará, 66075-110, Belém-PA, Brazil.; Brazil
 
2. Creator Author's name, affiliation, country SILVIA M. M. FAUSTINO; Bionorte Post-Graduate Program, UNIFAP, Federal University of Amapá, 68903-419, Macapá-AP, Brazil.; Brazil
 
2. Creator Author's name, affiliation, country VITOR RAMOS; CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, 4050-208 Matosinhos, Portugal.; Portugal
 
2. Creator Author's name, affiliation, country VITOR VASCONCELOS; CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, 4050-208 Matosinhos, Portugal. Department of Biology, Faculty of Sciences, University of Porto, 4069-007 Porto, Portugal.; Portugal
 
3. Subject Discipline(s)
 
3. Subject Keyword(s) Amazonia, Limnothrix, Polyphasic approach, Pseudanabaena, 16S rRNA phylogeny, Algae
 
3. Subject Subject classification Algae
 
4. Description Abstract

The Amazon region contains a great diversity of species, and the Amazon River basin accounts for almost 20% of all the freshwater in the world. Despite the favorable environmental conditions in this region, little is known about the cyanobacterial diversity of this waterbody, especially at the mouth of the river. In this paper, we used the polyphasic approach to identify 14 cyanobacterial strains isolated in the Amazon River on the inlet site from a drinking water supply located close to the river mouth. The isolated strains were characterized based on morphology, behavior in culture, 16S rRNA gene sequencing, phylogenetic analysis and potential for toxin production. The isolated strains belong to seven different genera, namely, Alkalinema, Cephalothrix, Limnothrix, Leptolyngbya, Phormidium, Pseudanabaena and an unidentified Nostocales taxa that may represent a new genus. Strikingly, there were no new species, nor detection of gene clusters associated with cyanotoxin production. However, the phylogenetic placements of the Amazonian strains of Limnothrix and Pseudanabaena provide new insight into the taxonomy of these genera, reinforcing the need for taxonomic revision.

 
5. Publisher Organizing agency, location Magnolia Press
 
6. Contributor Sponsor(s)
 
7. Date (YYYY-MM-DD) 2019-01-11
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF/A (4MB), Supplemental file (PDF)
 
10. Identifier Uniform Resource Identifier https://biotaxa.org:443/Phytotaxa/article/view/phytotaxa.387.4.1
 
10. Identifier Digital Object Identifier (DOI) http://dx.doi.org/10.11646/phytotaxa.387.4.1
 
11. Source Title; vol., no. (year) Phytotaxa; Vol 387, No 4: 11 January 2019
 
12. Language English=en en
 
13. Relation Supp. Files
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c) 2019 Phytotaxa