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DOI: http://dx.doi.org/10.11646/zootaxa.3780.3.2

Descriptions of three new species of Marcusenius Gill, 1862
(Teleostei: Mormyridae) from South Africa and Mozambique

PHOLOSHI A. MAAKE, OFER GON, ERNST R. SWARTZ

Abstract


Morphological and genetic studies of mormyrid fishes belonging to the genus Marcusenius from South Africa and Mozambique revealed four species of which three are described as new. Marcusenius pongolensis is widespread throughout the Incomati, Pongola and Kosi river systems, and sparsely represented in the Limpopo River system in South Africa. Marcusenius krameri sp. nov. is endemic to the Limpopo River system, and is the sister taxon of M. caudisquamatus sp. nov. from the Nseleni and Mhlatuze river systems in the KwaZulu-Natal Province of South Africa. The Ruvuma specimens were well differentiated from all southern and eastern African species of Marcusenius, and are therefore recognised as M. lucombesi sp. nov. Phylogenetic analysis of the mitochondrial cytochrome b gene showed that the three new species and M. altisambesi form a monophyletic lineage that is sister to a group containing M. pongolensis and M. macrolepidotus. Marcusenius krameri and M. lucombesi seems to prefer slow flowing reaches of rivers, whereas M. pongolensis and M. caudisquamatus seems to mainly inhabit rapid flowing sections of rivers with a rocky substrate. A key to the southern African species of Marcusenius is provided.


Keywords


DNA barcoding, cytochrome b, genetics, genetypes, identification key, Marcusenius, morphology, Mozambique, South Africa, species descriptions

References


Abuzinadah, O.A. (1995) Gill raker morphology in some Red Sea fishes of different feeding preferences. JKAU: Marine Science, 6, 93–122.
http://dx.doi.org/10.4197/mar.6-1.8

Alves-Gomes, J. & Hopkins, C.D. (1997) Molecular insights into the phylogeny of mormyriform fishes and the evolution of their electric organs. Brain, Behaviour and Evolution, 49, 324–351.
http://dx.doi.org/10.1159/000113001

Bastian, J. (1986) Electrolocation: behavior, anatomy and physiology. In: Bullock, T.H. & Heiligenberg, W. (Eds.), Electroreception. John Wiley & Sons, New York, pp. 577–612.

Bennett, M.V.L. (1971) Electroreception. In: Hoar, W.S. & Randall, D.J. (Eds.), Fish Physiology. Academic Press, London New York, pp. 493–574.

Bigorne, R. & Paugy, D. (1990) Description de Marcusenius meronai, espèce nouvelle de Mormyridae (Teleostei) de Sierra Leone. Ichthyological Exploration of Freshwaters, 1, 33–38.

Boden, G., Teugels, G.G. & Hopkins, C.D. (1997) A systematic revision of the large-scaled Marcusenius with description of a new species from Cameroon (Teleostei; Osteoglossomorpha; Mormyridae). Journal of Natural History, 31, 1645–1682.
http://dx.doi.org/10.1080/00222939700770881

Booth, A.J. & Khumalo, N. (2010) Age, growth and reproduction of Marcusenius pongolensis, Oreochromis mossambicus and Schilbe intermedius in an oligotrophic impoundment in Swaziland. African Journal of Ecology, 48, 481–489.
http://dx.doi.org/10.1111/j.1365-2028.2009.01136.x

Boulenger, G.A. (1898) Matériaux pour la faune du Congo. Poissons nouveaux du Congo. Première Partie. Mormyres. Annales du Musée du Congo, Zoologie, Série I, 1, 1–20.

Boulenger, G.A. (1899) A revision of the genera and species of the fishes of the family Mormyridae. Proceedings of the Zoological Society of London, 1898, 775–821.

Boulenger, G.A. (1905) Descriptions of four new freshwater fishes discovered by Dr. W. J. Ansorge in Angola. Annals and Magazine of Natural History, Ser. 7, 15 (89), 457–459.

Burridge, C.P., Craw, D., Fletcher, D. & Waters, J.M. (2008) Geological dates and molecular rates: Fish DNA sheds light on time dependency. Molecular Biology and Evolution, 25, 624–633.
http://dx.doi.org/10.1093/molbev/msm271

Carlson, B.A., Hasan, S.M., Hollmann, M., Miller, D.B., Harmon, L.J. & Arnegard, M.E. (2011) Brain evolution triggers increased diversification of electric fishes. Science, 332, 583–583.
http://dx.doi.org/10.1126/science.1201524

Chakrabarty, P. (2010) Genetypes: a concept to help intergrate molecular phylogenetics and taxonomy. Zootaxa, 2632, 67–68.

Crass, R.S. (1960) Notes on the freshwater fishes of Natal with descriptions of four new species. Annals of the Natal Museum, 14, 405–458.

Felsenstein, J. (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17, 368–376.
http://dx.doi.org/10.1007/bf01734359

Feulner, P.G.D., Kirschbaum, F., Mamonekene, V., Ketmaier, V. & Tiedemann, R. (2006) Adaptive radiation in African weakly electric fish (Teleostei: Mormyridae: Campylomormyrus): a combined molecular and morphological approach. Journal of Evolutionary Biology, 20, 403–414.
http://dx.doi.org/10.1111/j.1420-9101.2006.01181.x

Fowler, H.W. (1934) Fishes obtained by Mr. H.W. Bell-Marley chiefly in Natal and Zululand in 1929 to 1932. Proceedings of the Academy of Natural Sciences of Philadelphia, 86, 405–514.

Gilchrist, J.D.F. & Thompson, W.W. (1913) The freshwater fishes of South Africa. Annals of the South African Museum, XI, 321–463.

Gill, T.N. (1862). On the West African genus Hemichromis and descriptions of new species in the Museums of the Academy and Smithsonian Institution. Proceedings of the Academy of Natural Sciences of Philadelphia 14, 134–139.

Günther, A.C.L.G. (1866) Catalogue of the fishes in the British Museum. Catalogue of the Physostomi, containing the families Salmonidae, Percopsidae, Galaxidae, Mormyridae, Gymnarchidae, Esocidae, Umbridae, Scombresocidae, Cyprinodontidae, in the collection of the British Museum, volume 6. London, pp. 1–368.

Hammer, Ø., Harper, D. & Ryan, P.D. (2001) PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4, 9.

Hanika, S. & Kramer, B. (2000) Intra-male variability of its communication signal in the weakly electric fish, Marcusenius macrolepidotus (South African form), and possible functions. Behaviour, 142, 145–166.
http://dx.doi.org/10.1163/1568539053627677

Hebert, P.D.N., Cywinska, A., Ball, S.L. & De Waard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society London B, 270, 313–321.

Hopkins, C.D. (1981) On the diversity of electric signals in a community of mormyrid electricfish in West Africa. American Zoologist, 21, 211–222.

Hopkins, C.D. (1986) Behaviour of Mormyridae. In: Bullock T.H. & Heiligenberg, W. (Eds.), Electroreception. John Wiley & Sons, New York. pp. 527–576.

Hopkins, C.D. (2009) Electrical perception and communication. In: Squire L.R. (Ed.), Encyclopedia of Neuroscience, volume 3. Academic Press, Oxford, pp. 813–831.

Ivanova, N.V., Zemlak, T.S., Hanner, R.H. & Hebert, P.D.N. (2007) Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes, 7, 544–548.
http://dx.doi.org/10.1111/j.1471-8286.2007.01748.x

Kirschbaum, F. (1975) Development of the electric organ discharge in mormyrid and gymnotid fish (Marcusenius sp.and Eigenmannia virescens). Experientia, 1, 1290–1292.
http://dx.doi.org/10.1007/bf01945786

Kramer, C.Y. (1956) Extension of multiple range tests to group means with unequal number of replications. Biometrics, 12, 307–310.
http://dx.doi.org/10.2307/3001469

Kramer, B. (2013a) A morphological study on species of African Mormyrus (Teleostei: Mormyridae) and their electric organ discharges. African Journal of Aquatic Science, 38, 1–19.
http://dx.doi.org/10.1080/10236244.2013.796161

Kramer, B. (2013b) Differentiation in morphology and electrical signalling in four species of para- and sympatric Marcusenius (Teleostei: Mormyridae) from Côte d’Ivoire, West Africa. Marine and Freshwater Behaviour and Physiology, 46, 105–133.
http://dx.doi.org/10.2989/16085914.2012.745806

Kramer, B. & Swartz, E.R. (2010) A new species of slender Stonebasher within the Hippopotamyrus ansorgii complex from the Cunene River in southern Africa (Teleostei: Mormyridae). Journal of Natural History, 44, 2213–2242.
http://dx.doi.org/10.1080/00222931003764089

Kramer, B. & Van der Bank, F.H. (2000) The southern churchill, Petrocephalus wesselsi, a new species of mormyrid from South Africa defined by electric organ discharges, genetics, and morphology. Environmental Biology of Fishes, 59, 393–413.

Kramer, B. & Van der Bank, F.H. (2011) The Victoria Falls, a species boundary for the Zambezi Parrotfish, Cyphomyrus discorhynchus (Peters, 1852), and the resurrection of Cyphomyrus cubangoensis (Pellegrin, 1936) (Mormyridae: Teleostei). Journal of Natural History, 45, 2669–2699.
http://dx.doi.org/10.1080/00222933.2011.597945

Kramer, B. & Westby, G.W.M. (1985) No sex difference in the wave form of the pulse type electric fish, Gnathnemus petersii (Mormyridae). Experientia, 41, 1530–1531.
http://dx.doi.org/10.1007/bf01964790

Kramer, B. & Wink, M. (2013) East-west differentiation in the Marcusenius macrolepidotus species complex in southern Africa: the description of a new species for the lower Cunene River, Namibia (Mormyridae, Teleostei). Journal of Natural History, 47, 2327–2362.
http://dx.doi.org/10.1080/00222933.2013.798699

Kramer, B., Bills, R., Skelton, P. & Wink, M. (2012) A critical revision of the Churchill snoutfish, genus Petrocephalus, from southern and eastern Africa, with the recognition of P. tanensis, and the description of five new species (Teleostei, Mormyridae). Journal of Natural History, 46, 2179–2258.
http://dx.doi.org/10.1080/00222933.2012.708452

Kramer, B., Skelton, P.H., Van der Bank, F.H. & Wink, M. (2007) Allopatric differentiation in the Marcusenius macrolepidotus species complex in southern and eastern Africa: the resurrection of M. pongolensis and M. angolensis, and the description of two new species (Mormyridae, Teleostei). Journal of Natural History, 41, 647–708.
http://dx.doi.org/10.1080/00222930701250987

Kramer, B., Van der Bank, H., Flint, N., Sauer-Gürth H. & Wink M. (2003) Evidence for parapatric speciation in the mormyrid fish, Pollimyrus castelnaui (Boulenger, 1911), from the Okavango – Upper Zambezi river systems: P. marianne sp. nov., defined by electricorgan discharges, morphology and genetics. Environmental Biology of Fishes, 77, 47–70.

Kramer, B., Van der Bank, H. & Wink, M. (2013) Marked differentiation in a new species of dwarf stonebasher, Pollimyrus cuandoensis sp. nov. (Mormyridae: Teleostei), from a contact zone with two sibling species of the Okavango and Zambezi rivers. Journal of Natural History, 48 (7–8), 429–463.
http://dx.doi.org/10.1080/00222933.2013.807950

Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M, Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics, 23, 2947–2948.
http://dx.doi.org/10.1093/bioinformatics/btm404

Lavoué, S., Arnegard, M.E., Sullivan, J.P. & Hopkins, S.D. (2008) Petrocephalus of Odzala offer insights into evolutionary patterns of signal diversification in the Mormyridae, a family of weakly electrogenic fishes from Africa. Journal of Physiology-Paris, 102, 322–339.
http://dx.doi.org/10.1016/j.jphysparis.2008.10.003

Lissmann, H.W. (1951) Continuous electric signals from the tail of a fish Gymnarchus niloticus Curvier. Nature London, 167, 201–202.
http://dx.doi.org/10.1038/167201a0

Lissmann, H.W. & Machin, K.E. (1958) The mechanism of object location in Gymnarchus niloticus and similar fish. Journal of Experimental Biology, 35, 451–486.

Machordom, A. & Doadrio, I. (2001) Evidence of a Cenozoic Betic-Kabilian connection based on freshwater fish phylogeography (Luciobarbus, Cyprinidae). Molecular Phylogenetics and Evolution, 18, 252–263.
http://dx.doi.org/10.1006/mpev.2000.0876

McGarigal, K., Cushman, S. & Stafford, S. (2000) Multivariate statistics for wildlife and ecology research. Springer Verlag, New York, 283 pp.

Moore, A.E. & Larkin, P.A. (2001) Drainage evolution in south-central Africa since the breakup of Gondwana. South African Journal of Geology, 104, 47–68.
http://dx.doi.org/10.2113/104.1.47

Moritz, T., Engelmann, J., Linsenmair, K.E., & Von der Emde, G. (2009) The electric organ discharges of the Petrocephalus species (Teleostei: Mormyridae) of the Upper Volta system. Journal of Fish Biology, 74, 54–76.
http://dx.doi.org/10.1111/j.1095-8649.2008.02111.x

Peters, W.C.H. (1852) Einige neue Säugethiere und Flussfische aus Mossambique. Monatsberichte der Königlich-Preussischen Akademie der Wissenschaften Berlin, 1852, 273–276.

Pezzanite, B. & Moller, P. (1998) A sexually dimorphic basal anal-fin ray expansion in the weakly discharging electric fish Gnathonemus petersii. Journal of Fish Biology, 53, 638–644.
http://dx.doi.org/10.1111/j.1095-8649.1998.tb01007.x

Posada, D. & Crandall, K.A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
http://dx.doi.org/10.1093/bioinformatics/14.9.817

SAS Institute Inc. (2007) JMP Statistics and Graphic Guide. Cary, NC, USA, 1076 pp.

Sabaj Pérez, M.H. (Ed) (2013) Standard symbolic codes for institutional resource collections in herpetology and ichthyology: an Online Reference. Version 4.0 (28 June 2013) American Society of Ichthyologists and Herpetologists, Washington, DC. Available from: http://www.asih.org/node/204 (accessed 16 September 2013)

Skelton, P.H. (2001) A complete guide to the freshwater fishes of southern Africa. 2nd ed. Struik Publishers, Cape Town, South Africa, pp. 92–101.

Stankiewicz, J. & de Wit, M.J. (2006) A proposed drainage evolution model for Central Africa – Did the Congo flow east? Journal of African Earth Science, 44 (1), 75–84.

Sullivan, J.P. & Hopkins, C.D. (2005) A new Stomatorhinus (Osteoglossomorpha: Mormyridae) from the Ivindo River, Gabon, West Central Africa. Zootaxa, 847, 1–23.

Swofford, D.L. (2002) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinaeur Assoc Inc., Sunderland, Massachusetts.

Szabo,T. & Fessard,A. (1974) Physiology of electroreceptors. In: Fessard, A. (Ed.), Handbook of Sensory Physiology. Springer-Verlag, Berlin Heidelberg New York, pp. 59–124.

Taverne, L. (1971) Ostéologie des genres Marcusenius Gill, Hippopotamyrus Pappenheim, Cyphomyrus Myers, Pollimyrus Taverne et Brienomyrus Taverne (Pisces Mormyriformes). Annales Musée Royal de l’Afrique Centrale, Sciences Zoologiques, 188, 144.

Taverne, L. (1972) Ostéologie des genres Mormyrus Linné, Mormyrops Müller, Hyperopisus Gill, Isichthys Gill, Myomyrus Boulenger, Stomatorhinus Boulenger et Gymnarchus Cuvier. Considérations générales sur la systématique des poissons de l’ordre des Mormyriformes. Annales Musée Royal de l’Afrique Centrale, Sciences Zoologiques, 200, 1–194.

Tukey, J.W. (1953) The problem of multiple comparisons. Princeton University, N.J., 396 pp. [unpublished manuscript]

Werneyer, M. & Kramer, B. (2005) Electric signalling and reproductive behaviour in a mormyrid fish, the bulldog Marcusenius macrolepidotus (South African form). Japan Ethology, 23, 113–125.
http://dx.doi.org/10.1007/s10164-004-0136-0


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