Open Access Open Access  Restricted Access Subscription or Fee Access


Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae)



Cobitis avicennae, new species, from the Karkheh and Karun sub-drainages in the Tigris catchment is distinguished from other Cobitis species in the Persian Gulf, Kor and the southern Caspian Sea basins by having a single lamina circularis in males, a small comma-shaped black spot on the upper caudal-fin base, 5½ branched anal-fin rays, 5–6 rows of dark spots on the dorsal and caudal fins, scales below the dorsal-fin base with a small focal zone and pigmentation zone Z4 with 12–17 large, partly fused blotches. It is also distinguished from other Cobitis species in the comparison group by six fixed, diagnostic nucleotide substitutions in the mtDNA COI barcode region.


Cobitis, morphology, new species, Tigris, Iran

Full Text:

PDF/A (6.36 MB)


Abdoli, A. (2000) The inland water fishes of Iran. Iranian Museum of Nature and Wildlife, Tehran, 378 pp. [In Persian].

Biomatters (2013) Geneious Pro. Available: (Accessed 25 Jan. 2015)

Bohlen, J., Perdices, A., Doadrio, I. & Economidis, P.S. (2006) Vicariance, colonisation, and fast local speciation in Asia Minor and the Balkans as revealed from the phylogeny of spined loaches (Osteichthyes; Cobitidae). Molecular Phylogenetics and Evolution, 39 (2), 552–561.

Coad, B.W. & Sarieyyiipoglu, M. (1988) Cobitis elazigensis, a new species of cobitid fish from Anatolia, Turkey. Japanese Journal of Ichthyology, 34, 426–430.

Coad, B.W. (2010) Freshwater fishes of Iraq. Pensoft Publishers, Sofia, 294 pp.

Edgar, R.C. (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792– 1797.

Erk'akan, F., Atalay-Ekmekçi, F.G. & Nalbant, T.T. (1998) Four new species and one new subspecies of the genus Cobitis (Pisces: Ostariophysi: Cobitidae) from Turkey. Turkish Journal of Zoology, 22, 9–15.

Esmaeili, H.R., Coad, B.W., Gholamifard, A., Nazari, N. & Teimori, A. (2010) Annotated checklist of the freshwater fishes of Iran. Zoosystematica Rossica, 19, 361–386.

Gambetta, L. (1934) Sulla variabilita del cobite fluviale (Cobitis taenia L.) e sul rapporto numerico dei sessi. Bollettino dei Musei di Zoologia e di Anatomia Comparata della Reale Università di Torino, 44, 297–324.

Ivanova, N.V., Zemlak, T.S., Hanner R.H. & Hebert, P.D.N. (2007). Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes, 7, 544–548.

Kottelat, M. & Freyhof, J. (2007) Handbook of European freshwater fishes. Kottelat, Cornol and Freyhof, Berlin, xiv+646 pp.

Kottelat, M. (2012) Conspectus cobitidum: an inventory of the loaches of the world (Teleostei: Cypriniformes: Cobitoidei). The Raffles Bulletin of Zoology, suppl. 26, 1–199.

Meier, R., Kwong, S., Vaidya, G., Ng, P. & Peter K.L. (2006) DNA barcoding and taxonomy in Diptera: a tale of high intraspecific variability and low identification success. Systematic Biology, 55, 715-728.

Mousavi-Sabet, H., Vasil'eva, E.D., Vatandoust, S. & Vasil'ev, V.P. (2011) Cobitis faridpaki sp. nova — a new spined loach species (Cobitidae) from the Southern Caspian Sea Basin (Iran). Journal of Ichthyology, 51, 925–931.

Mousavi-Sabet, H., Yerli, S.V., Vatandoust, S., Ozeren, S.C. & Moradkhani, Z. (2012) Cobitis keyvani sp. nova—a new species of spined-loach from south of the Caspian Sea basin (Teleostei: Cobitidae). Turkish Journal of Fisheries and Aquatic Sciences, 12, 7–13.

Perdices, A. & Doadrio, I. (2001) The molecular systematics and biogeography of the European cobitids based on mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 19 (3), 468–478.

Perdices, A., Doadrio, I., Economidis, P.S., Bohlen, J. & Banarescu, P. (2003) Pleistocene effects on the European freshwater fish fauna: double origin of the cobitid genus Sabanejewia in the Danube basin (Osteichthyes: Cobitidae). Molecular Phylogenetics and Evolution, 26 (2), 289–299.

Perdices, A., Bohlen, J. & Doadrio, I. (2008) The molecular diversity of Adriatic spined loaches (Teleostei, Cobitidae). Molecular Phylogenetics and Evolution, 46 (1), 382–390.

Posada, D. & Crandall, K.A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14, 817–818.

Saitou, N. & Nei, M. (1987) The Neighbor-Joining Method – a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406-425.

Swofford, D.L. (2002) PAUP*. Phylogenetic Analysis Using Parsimony (* and Other Methods). Version 4. Sinauer Associates, Sunderland.

Tamura, K. (1992) Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9, 678-687.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739.

Vasil’eva, E.D. & Vasil’ev, V.P. (2012) Cobitis amphilekta sp. nova, a new species of spined loaches (Cobitidae, Cypriniformes) from the Caspian Sea basin. Journal of Ichthyology, 52, 200–206.


  • There are currently no refbacks.

ISSN 1175-5326 (Print Edition) & ISSN 1175-5334 (Online Edition)
Published by Magnolia Press, Auckland, New Zealand
A founding journal of Biotaxa