Open Access Open Access  Restricted Access Subscription or Fee Access


DOI: http://dx.doi.org/10.11646/zootaxa.3946.1.3

Two new species of Amazophrynella (Amphibia: Anura: Bufonidae) from Loreto, Peru

ROMMEL R. ROJAS, VINÍCIUS TADEU DE CARVALHO, Robson W. ÁvilA, IZENI PIRES FARIAS, MARCELO GORDO, TOMAS HRBEK

Abstract


Amazophrynella is a taxonomically poorly known bufonid genus with a pan-Amazonian distribution. A large part of this ambiguity comes from taxonomic uncertainties regarding the type species A. minuta. In this study we compare morphological and molecular data of topotypic specimens of A. minuta with all other nomical congeneric species. Based on these comparisons, we describe two new species. The first species, A. amazonicola sp. nov., differs from other recognized congeners by having a tip of snout with a small triangular protrusion (in dorsal and lateral view), spiculated body and basal webbing on fingers I and II. The second species, A. matses sp. nov., differs from congeners by the smallest snout to vent length of the genus, edges of nasal protrusion dilated and elliptical shape palmar tubercles. The two species are allopatric, where the first species is known to be associated with white sand forests (=campinaranas), while the second inhabits upland (=terra firme) forests. Both species are diagnosable by a series of substitutions in the 16S rDNA, and both species are highly divergent from their sister taxa (p-distances range from 7–14%)

 


Keywords


Amazon basin, Riverine barriers, Biodiversity, Speciation

Full Text:

PDF/A (23.97 MB)

References


Ávila, R.W., Carvalho, V.T., Gordo, M., Ribeiro, K. & Morais, D. (2012) A new species of Amazophrynella (Anura: Bufonidae) from southern Amazonia. Zootaxa, 3484, 65–74.

Baum, D.A. & Donoghue, M.J. (1995) Choosing among alternative “phylogenetic” species concepts. Systematic Botany, 20, 560–573.
http://dx.doi.org/10.2307/2419810

Brown, J. & Twomey, E. (2009) Complicated histories: three new species of poison frogs of the genus Ameerega (Anura: Dendrobatidae) from north-central Peru. Zootaxa, 2049, 1–38.

Cracraft, J. (1983) Species concepts and speciation analysis. Current Ornithology, 1, 159–187.
http://dx.doi.org/10.1007/978-1-4615-6781-3_6

Crawford, A.J., Lips, K.R. & Bermingham, E. (2010) Epidemic disease decimates amphibian abundance, species diversity, and evolutionary history in the highlands of central Panama. Proceedings of the National Academy of Sciences of the United States of America, 107, 13777–13782.
http://dx.doi.org/10.1073/pnas.0914115107

Cruz, C.A.G. & Fusinatto, L.A. (2008) A new species of Dendrophryniscus Jiménes de la Espada, 1871 (Amphibia, Anura, Bufonidae) from the Atlantic Forest of Rio Grande do Sul, Brazil. South American Journal of Herpetology, 3, 22–26.
http://dx.doi.org/10.2994/1808-9798(2008)3[22:ansodj]2.0.co;2

Davis, J.I. & Nixon, K.C. (1992) Populations, genetic variation, and the delimitation of phylogenetic species. Systematic Biology, 41, 421–435.
http://dx.doi.org/10.1093/sysbio/41.4.421

Darst, C.R. & Cannatella, D.C. (2004) Novel relationships among hyloid frogs inferred from 12S and 16S mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 31, 462–475.
http://dx.doi.org/10.1016/j.ympev.2003.09.003

de Queiroz, K. (2007) Species concepts and species delimitation. Systematic Biology, 56, 879–886.
http://dx.doi.org/10.1080/10635150701701083

Duellman, W.E. & Mendelson, J.R. (1995) Amphibians and reptiles from northern Departamento Loreto, Peru: Taxonomy and biogeography. University of Kansas Science Bulletin, 55, 329–376.

Duellman, W.E. (1978) The biology of an equatorial herpetofauna in Amazonian Ecuador. Miscellaneous Publications, University of Kansas Museum of Natural History, 65, 1–352.

Felsenstein, J. (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17, 396–376.
http://dx.doi.org/10.1007/bf01734359

Felsenstein, J. (1981) Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783–791.
http://dx.doi.org/10.2307/2408678

Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS ONE, 2, e1109.
http://dx.doi.org/10.1371/journal.pone.0001109

Fouquet, A., Recoder, R., Teixeira Jr., M., Cassimiro, J., Amaro, R.C., Camacho, A., Damasceno, R., Carnaval, A.C., Moritz, C. & Rodrigues, M.T. (2012a) Molecular phylogeny and morphometric analyses reveal deep divergence between Amazonia and Atlantic Forest species of Dendrophryniscus. Molecular Phylogenetics and Evolution, 62, 823–838.
http://dx.doi.org/10.1016/j.ympev.2011.11.023

Fouquet, A., Recoder, R., Teixeira Jr., M., Cassimiro, J., Amaro, R.C., Camacho, A., Damasceno, R., Carnaval, A.C., Moritz, C. & Rodrigues, M.T. (2012b) Amazonella Fouquet et al.2012 (Anura: Bufonidae) junior homonym of Amazonella Lundblad, 1931 (Acari: Unionicolidae): proposed replacement by Amazophrynella nom. nov. Zootaxa, 3244, 68.

Fouquet, A., Ledoux, J.B., Dubut, V., Noonan, B.P. & Scotti, I. (2012c) The interplay of dispersal limitation, rivers, and historical events shapes the genetic structure of an Amazonian frog. Biological journal of the Linnean Society, 106, 356–337.
http://dx.doi.org/10.1111/j.1095-8312.2012.01871.x

Fouquet, A., Noonan, B.P., Rodrigues, M.T., Pech, N., Gilles, A. & Gemmell, N.J. (2012d) Multiple quaternary refugia in the eastern Guiana Shield revealed by comparative phylogeography of 12 frog species. Systematic Biology, 61, 1–28.
http://dx.doi.org/10.1093/sysbio/syr130

Frost, D.R. (2014) Amphibian Species of the World: an Online Reference. Version 5.4 (9 January, 2013) American Museum of Natural History, New York, USA. Available from: http://research.amnh.org/herpetology/amphibia/ (acessed 10 January 2015)

Gordo, M. (2006) In: Vriesendorp, C., Pitman, N., Rojas, M., Pawlak, B.A., Rivera, C., Calixto M., Vela C.P. & Fasabi, R. (Eds.), 2006. Perú: Matsés. Rapid Biological Inventories Report 16. The Field Museum, Chicago, Illinois, 336 pp.

Izecksohn, E. (1993) Nova espécie de Dendrophryniscus da região amazônica (Amphibia, Anura, Bufonidae). Revista Brasileira de Zoologia, 10, 407–412.
http://dx.doi.org/10.1590/s0101-81751993000300006

Jobb, G. (2008) TREEFINDER version of March 2011. Distributed by the author, Munich, Germany. Available from: http://www.treefinder.de/ (accessed 27 May 2013)

Kok, P.J. & Kalamandeen, M. (2008) Introduction to the taxonomy of the amphibians of Kaieteur National Park, Guyana. Abc Taxa Volumen 5, 288 pp.

Lima, A.P., Magnusson, W.E., Menin, M., Erdtmann, L.K., Rodrigues, D.J., Keller, C. & Hödl, W. (2006) Guia de sapos da Reserva Adolpho Ducke, Amazônia Central – Guide to the frogs of Reserva Adolpho Ducke, Central Amazonian. Áttema Design Editorial, Manaus, 168 pp.

Magnusson, W.E. & Hero, J.H. (1991) Predation and the evolution of complex oviposition behaviour in Amazon rainforest frogs. Oecologia, 86, 310–318.
http://dx.doi.org/10.1007/bf00317595

Melin, D.E. (1941) Contributions to the knowledge of the Amphibia of South America. Göteborgs Kungl. Vetenskaps– och Vitterhets–samhälles. Handlingar. Serien B, Matematiska och Naturvetenskapliga Skrifter, 1, 1–71.

Padial, J.M. & De la Riva, I. (2009) Integrative taxonomy reveals cryptic Amazonian species of Pristimantis (Anura: Strabomantidae). Zoological Journal of the Linnean Society, 155, 97–122.

Padial, J.M., Miralles, A., De la Riva, I. & Vences, M. (2010) The integrative future of taxonomy. Frontiers in Zoology, 7, 16.
http://dx.doi.org/10.1186/1742-9994-7-16

Padial, J.M., Chaparro, J.C., Castro Viejo-Fisher, S., Guyasamin, J., Lehr, E., Delgado, A., Vaira, M., Texeira, J.R., Aguayo, R. & De la Riva, I. (2012) A revision of species diversity in the Neotropical genus Oreobates (Anura: Strabomantidae), with the description of three new species from the Amazonian slopes of the Andes. Americam Museum Novitates, 3755, 1–55.
http://dx.doi.org/10.1206/3752.2

Posada, D. (2006) ModelTest Server: a web-based tool for the statistical selection of models of nucleotide substitution online. Nucleic Acids Research, 1, 34.
http://dx.doi.org/10.1093/nar/gkl042

Pramuk, J.B., Robertson, B., Sites, J.W. & Noonan, B.P. (2008) Around the world in 10 million years: biogeography of the nearly cosmopolitan true toads (Anura: Bufonidae). Global Ecology and Biogeography, 17, 72–83.
http://dx.doi.org/10.1111/j.1466-8238.2007.00348.x

Rach, J., DeSalle, R., Sarkar, I.N., Schierwater, B. & Hadrys, H. (2008) Character-based DNA barcoding allows discriminations of genera, species and populations in Odonata. Proceedings of the Royal Society of London, 275, 237–247.
http://dx.doi.org/10.1098/rspb.2007.1290

Rodríguez, L.O. & Duellman, W.E. (1994) Guide to the Frogs of the Iquitos Region. Amazonian Peru. University of Kansas Natural History Museum, special Publications, 22, 1–80.

Rojas, R.R., Carvalho, V.T., Gordo, M., Ávila, R.W., Farias, I.P. & Hrbek, T. (2014) A new species of Amazophrynella (Anura: Bufonidae) from the southwestern part of the Brazilian Guiana Shield. Zootaxa, 3753 (1), 79–95.
http://dx.doi.org/10.11646/zootaxa.3753.1.7

Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, second edition. Cold Spring Harbor Laboratory Press, Cold Springs Harbor, NY, 1626 pp.

Simões, P.I., Lima, A.P., Magnusson, W.E., Hödl, W. & Amézquita, A. (2008) Acoustic and morphological differentiation in the frog Allobates femoralis: relationships with the upper Madeira River and other potential geological barriers. Biotropica, 40, 607–614.
http://dx.doi.org/10.1111/j.1744-7429.2008.00416.x

Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.
http://dx.doi.org/10.1093/molbev/msm092

Vences, M., Meike, T., Van der Meijden, A., Chiari, Y. & Vieites, D. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2, 7–12.


Refbacks

  • There are currently no refbacks.


 
ISSN 1175-5326 (Print Edition) & ISSN 1175-5334 (Online Edition)
Published by Magnolia Press, Auckland, New Zealand
A founding journal of Biotaxa