Open Access Open Access  Restricted Access Subscription or Fee Access


DOI: http://dx.doi.org/10.11646/zootaxa.3994.3.2

Molecular phylogeny of Niphargus boskovici (Crustacea: Amphipoda) reveals a new species from epikarst

VID ŠVARA, TEO DELIĆ, TONĆI RAĐA, CENE FIŠER

Abstract


We describe a new species of an amphipod Niphargus zagorae sp. n. and redescribe its nearest relative and morphologically similar species N. boskovici S. Karaman, 1952. We present the geographic distributions of both species, morphological diagnoses and infer their phylogenetic position within the genus based on COI, 28S and H3 markers.

 


Keywords


Niphargus, taxonomy, cryptic species, epikarst

Full Text:

PDF/A (924.12 KB)

References


Bilandžija, H., Morton, B., Podnar, M. & Ćetković, H. (2013) Evolutionary history of relict Congeria (Bivalvia: Dreissenidae): unearthing the subterranean biodiversity of the Dinaric Karst. Frontiers in Zoology, 10 (5), 1–17.
http://dx.doi.org/10.1186/1742-9994-10-5

Coleman, C.O. (2003) "Digital inking": How to make perfect line drawings on computers. Organisms Diversity and Evolution, Electronic Supplement, 14, 1–14.
http://dx.doi.org/10.1078/1439-6092-00081

Coleman, C.O. (2009) Drawing setae the digital way. Zoosystematics and Evolution, 85, 305–310.
http://dx.doi.org/10.1002/zoos.200900008

Colgan, D.J., McLauchlan, A., Wilson, G.D.F., Livingston, S.P., Edgecombe, G.D., Macaranas, J., Cassis, G. & Gray, M.R. (1998) Histone H3 and U2 snRNA DNA sequences and arthropod molecular evolution. Australian Journal of Zoology, 46, 419–437.
http://dx.doi.org/10.1071/ZO98048

Cothran, R.D., Stiff, A.R., Chapman, K., Wellborn, G.A. & Relyea, R.A. (2013) Reproductive interference via interspecific pairing in an amphipod species complex. Behavioral Ecology and Sociobiology, 67, 1357–1367.
http://dx.doi.org/10.1007/s00265-013-1564-z

Culver, D.C. & Pipan, T. (2014) Shallow Subterranean Habitats. Ecology, Evolution, and Conservation. Oxford University Press, Oxford, 288 pp.

Fišer, C. (2012) Niphargus: a model system for evolution and ecology. In: Culver, D.C. &White, W.B. (Eds.), Encyclopedia of Caves. Academic Press, New York, pp. 555–564.

Fišer, C., Sket, B. & Trontelj, P. (2008a) A phylogenetic perspective on 160 years of troubled taxonomy of Niphargus (Crustacea: Amphipoda). Zoological Scripta, 37, 665–680.

Fišer, C., Bininda-Emonds, O.P.R., Blejec, A. & Sket, B. (2008a) Can heterochrony help explain the high morphological diversity within the genus Niphargus (Crustacea: Amphipoda)? Organisms, Diversity and Evolution, 8, 146–162.
http://dx.doi.org/10.1016/j.ode.2007.06.002

Fišer, C., Coleman, C.O., Zagmajster, M., Zwittnig, B., Gerecke, R. & Sket, B. (2010) Old museum samples and recent taxonomy: A taxonomic, biogeographic and conservation perspective of the Niphargus tatrensis species complex (Crustacea: Amphipoda). Organisms Diversity and Evolution, 10, 5–22.
http://dx.doi.org/10.1007/s13127-010-0006-2

Fišer, C., Trontelj, P., Luštrik, R. & Sket, B. (2009b) Toward a unified taxonomy of Niphargus (Crustacea: Amphipoda): a review of morphological variability. Zootaxa, 2061, 1–22.

Fišer, C. & Zagmajster, M. (2009) Cryptic species from cryptic space: the case of Niphargus fongi sp. n. (Amphipoda: Niphargidae). Crustaceana, 82, 593–614.
http://dx.doi.org/10.1163/156854009X407704

Folmer, O.M., Black, M., Hoeh, R., Lutz, R. & Vrijehoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 5, 304–313.

Karaman, G.S. (1973) XLVII Contribution to the knowledge of the Amphipoda. Two new Niphargus species from Crna gora (Montenegro), N. inclinatus n. sp. and N. boskovici alatus n. ssp. Periodicum biologorum, 75, 275–283.

Karaman, G.S. & Ruffo, S. (1986) Amphipoda: Niphargus-Group (Niphargidae sensu Bousfield, 1982). In: Botosaneanu, L. (Ed.), Stygofauna Mundi, A Faunistic, Distributional, and Ecological Synthesis of the World Fauna inhabiting Subterranean Warers (including the Marine Interstitial). E. J. Brill/ Dr. W. Backhuys, Leiden, pp. 514–534.

Karaman, G.S. (2014a) New member of the family Niphargidae from Croatia, Niphargus radzai, sp. n. (Contribution to the Knowledge of the Amphipoda 273). Zootaxa, 3811 (4), 585–598.
http://dx.doi.org/10.11646/zootaxa.3811.4.9

Karaman, G.S. (2014b) Further studies of genus Niphargus schiödte, 1849 (fam. Niphargidae) from Western Balkan Peninsula (contribution to the knowledge of the Amphipoda 274). Agriculture & Forestry, 60, 203–220.

Karaman, G.S. (2014c) New data on Niphargus boskovici S. Karaman, 1952 and some other Gammaridean amphipods from the western Balkans (Contribution to the knowledge of the Amphipoda 279). Biologia Serbica, 36, 39–54.

Karaman, S. (1952) Prilozi poznavanju nifarga Hercegovine i južne Dalmacije. Prirodoslovna istraživanja, Academia Scientiarum et Artium Slavorum Meridionalium, 25, 45–55.

Karaman, S. (1953) Über subterrane Amphipoden und Isopoden des Karstes von Dubrovnik und Seines Hinterlandes. Acta Musei Macedonici Scientarium Naturalium, 1, 137–167.

Karaman, S. (1960) Weitere Beiträge zur Kenntnis des jugoslavischen Niphargiden. Glasnik prirodnjačkog muzeja u Beogradu B, 15, 75–90.

Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability.Molecular Biology and Evolution, 30, 772–780.
http://dx.doi.org/10.1093/molbev/mst010

Lagrue, C, Wattier, R, Galipaud, M, Gauthey, Z, Rullman, J.P., Dubreuil, C., Rigaud, T. & Bollache, L. (2014) Confrontation of cryptic diversity and mate discrimination within Gammarus pulex and Gammarus fossarum species complexes. Freshwater Biology, 59, 2555–2570.
http://dx.doi.org/10.1111/fwb.12453

Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695–1701.
http://dx.doi.org/10.1093/molbev/mss020

Lefébure, T., Douady, C.J., Gouy, M., Trontelj, P., Briolay, J. & Gibert, J. (2006) Phylogeography of a subterranean amphipod reveals cryptic diversity and dynamic evolution in extreme environments. Molecular Ecology, 15, 1797–1806.
http://dx.doi.org/10.1111/j.1365-294X.2006.02888.x

Lefébure, T., Douady, C.J., Malard, F. & Gibert, J. (2007) Testing dispersal and cryptic diversity in a widely distributed groundwater amphipod (Niphargus rhenorhodanensis). Molecular Phylogenetics and Evolution, 42, 676–686.
http://dx.doi.org/10.1016/j.ympev.2006.08.020

McInerney, C.E., Maurice, L., Robertson, A.L., Knight, L.R.F.D., Arnscheidt, J., Venditti, C., Dooley, J.S.G., Mathers, T., Matthijs, S., Erikkson, K., Proudlove, G.S. & Hänfling, B. (2014) The Ancient Britons: Groundwater fauna survived extreme climate changes over tens of millions of years across NW Europe. Molecular Ecology, 23, 1153–1166.
http://dx.doi.org/10.1111/mec.12664

Meleg, I.N., Zakšek, V., Fišer, C., Kelemen, B.S. & Moldovan, O.T. (2013) Can Environment Predict Cryptic Diversity? The Case of Niphargus Inhabiting Western Carpathian Groundwater. PLoS ONE, 8, e76760.
http://dx.doi.org/10.1371/journal.pone.0076760

Mihevc, A., Prelovšek, M. & Zupan, N. (2010) Introduction to the Dinaric Karst. ZRC SAZU, Ljubljana, pp. 1–71.

Miller , M.A., Holder, M.T., Vos, R., Midford, P.E., Liebowitz, T., Chan, L., Hoover, P. & Warnow, T. (2012) The CIPRES portals CIPRES. Available from: http://www.phylo.org/sub_sections/portal (accessed 16 June 2015)

Padial, J.M., Miralles, A., De la Riva, I. & Vences, M. (2010) The integrative future of taxonomy. Frontiers in Zoology, 7, 16. [2010]
http://dx.doi.org/10.1186/1742-9994-7-16

Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
http://dx.doi.org/10.1093/bioinformatics/btg180

Schlick-Steiner, B.C., Steiner, F.M., Seifert, B., Stauffer, C., Christian, E. & Crozier, R.H. (2010) Integrative Taxonomy: A Multisource Approach to Exploring Biodiversity. Annual Review of Entomology, 55, 421–438.
http://dx.doi.org/10.1146/annurev-ento-112408-085432

Silvestro, D. & Michalak, I. (2012) raxmlGUI: a graphical front-end for RaxML. Organisms Diversity and Evolution, 12, 335–337.
http://dx.doi.org/10.1007/s13127-011-0056-0

Sket, B. (1999) High biodiversity in hypogean waters and its endangerment—situation in Slovenia, Dinaric karst, and Europe. Crustaceana, 72, 767–779.
http://dx.doi.org/10.1163/156854099503951

Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics, 22, 2688–2690.
http://dx.doi.org/10.1093/bioinformatics/btl446

Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
http://dx.doi.org/10.1093/molbev/mst197

Trontelj, P., Douady, C.J., Fišer, C., Gibert, J., Gorički, Š., Lefébure, T., Sket, B. & Zakšek, V. (2009) A molecular test for cryptic diversity in groundwater: how large are the ranges of macrostygobionts? Freshwater Biology, 54, 727–744.
http://dx.doi.org/10.1111/j.1365-2427.2007.01877.x

Trontelj, P., Blejec, A. & Fišer, C. (2012) Ecomorphological convergence of cave communities. Evolution, 66, 3852–3865.
http://dx.doi.org/10.1111/j.1558-5646.2012.01734.x

Väinölä, R., Witt, J.D.S., Grabowski, M., Bradbury, J.H., Jadzewski, K. & Sket, B. (2008) Global diversity of amphipods (Amphipoda; Crustacea) in freshwater. Hydrobiologia, 595, 241–255.
http://dx.doi.org/10.1007/s10750-007-9020-6

Verovnik, R., Sket, B. & Trontelj, P. (2005) The colonization of Europe by the freshwater crustacean Asellus aquaticus (Crustacea: Isopoda) proceeded from ancient refugia and was directed by habitat connectivity. Molecular Ecology, 14, 4355–4369.
http://dx.doi.org/10.1111/j.1365-294X.2005.02745.x

Zagmajster, M., Eme, D., Fišer, C., Galassi, D., Marmonier, P., Stoch, F., Cornu, J.-F. & Malard, F. (2014) Geographic variation in range size and beta diversity of groundwater crustacean: insights from habitats with low thermal seasonality. Global ecology and biogeography, 23, 1135–1145.
http://dx.doi.org/10.1111/geb.12200

Zakšek, V., Sket, B. & Trontelj, P. (2007) Phylogeny of the cave shrimp Troglocaris: evidence of a young connection between Balkans and Caucasus. Molecular Phylogenetics and Evolution, 42, 223–235.
http://dx.doi.org/10.1016/j.ympev.2006.07.009


Refbacks

  • There are currently no refbacks.


 
ISSN 1175-5326 (Print Edition) & ISSN 1175-5334 (Online Edition)
Published by Magnolia Press, Auckland, New Zealand
A founding journal of Biotaxa