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DOI: http://dx.doi.org/10.11646/zootaxa.3999.4.7

No millipede endemics north of the Alps? DNA-Barcoding reveals Glomeris malmivaga Verhoeff, 1912 as a synonym of G. ornata Koch, 1847 (Diplopoda, Glomerida, Glomeridae)

THOMAS WESENER

Abstract


In order to evaluate the status of the only species of pill millipede (Glomerida) endemic to Germany, Glomeris malmivaga Verhoeff, 1912, a DNA barcoding study based on the COI mitochondrial gene was conducted. Sequences of G. malmivaga were compared to those of G. ornata Koch, 1847 from Slovenia, of which the former was previously described as a variety of the latter before being elevated to subspecies- and, recently, species-rank. Included in the analysis were specimens of G. helvetica Verhoeff, 1894, also originally described as a variety of G. ornata, which was supposed to be closely related to G. malmivaga based on its morphology, as well as geographical proximity of occurrence. Additionally, G. valesiaca Rothenbühler, 1899, which occurs in sympatry and looks quite similar to G. helvetica was also sequenced for the first time and included in the study. Sequences of four widespread Glomeris species, all occurring in close proximity to G. malmivaga, G. marginata Villers, 1789, G. connexa Koch, 1847, G. klugii Brandt, 1833 and G. intermedia Latzel, 1884 were downloaded from Genbank and incorporated in the analysis. While G. helvetica and G. valesiaca were found to be clearly separate from G. ornata (11.8–14.6% p-distance), G. malmivaga is almost identical to the latter (0.5% p-distance), despite the large geographical distance between both species. Because of their great morphological and genetical similarity, G. malmivaga n. syn. is synonymised under G. ornata.

 


Keywords


Myriapoda, soil arthropod, endemism, central Europe, Barcoding, COI

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References


Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389–3402.
http://dx.doi.org/10.1093/nar/25.17.3389

Astrin, J.J. & Stüben, P.E. (2008) Phylogeny in cryptic weevils: molecules, morphology and new genera of western Palaearctic Cryptorhynchinae (Coleoptera: Curculionidae). Invertebrate Systematics, 22, 503–522.
http://dx.doi.org/10.1071/IS07057

Attems, C. (1949) Die Myriopoden Steiermarks. Sitzungsberichte der Österreichischen Akademie der Wissenschaften in Wien, mathematisch-naturwissenschaftliche Klassen, Abteilung I, 104, 117–238.

Brandt, J.F. (1833) Tentaminum quorundam monographicorum Insecta Myriapoda Chilognathi Latreillii spectantium prodromus. Bulletin de la Societé Impériale des Naturalistes de Moscou, 6, 194–209.

Ceuca, T. (1992) Quelques aspects sur la faunistique l'Ecologie et la zoogeographie des diplopodes de la Region Balkanique. In: Meyer, E., Thaler, K. & Schedl, W. (Eds.), Advances in Myriapodology. Berichte des naturwissenschaftlich-medizinischen Vereins in Innsbruck, 10 (Supplement), pp. 411–429.

Felsenstein, J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783–791.
http://dx.doi.org/10.2307/2408678

Foddai, D., Minelli, A., Scheller, U. & Zapparoli, M. (1995) Chilopoda, Diplopoda, Pauropoda, Symphyla. In: Minelli, A., Ruffo, S. & La Posta, S. (Eds.), Checklist delle Specie della Fauna Italiana, 32/33, pp. 1–35.

Golovatch, S.I., Geoffroy, J.J. & Mauriès, J.-P. (2006) Review of the millipede genus Hyleoglomeris Verhoeff, 1910 (Diplopoda, Glomerida, Glomeridae), with descriptions of new species from caves in Southeast Asia. Zoosystema, 28 (4), 887–915.

Golovatch, S.I., Mauriès, J.-P., Akkari, N., Stoev, P. & Geoffroy, J.J. (2009) The millipede genus Glomeris Latreille, 1802 (Diplopoda, Glomerida, Glomeridae) in North Africa. Zookeys, 12, 47–86.
http://dx.doi.org/10.3897/zookeys.12.179

Hoess, R., Scholl, A. & Lörtscher, M. (1997) The Glomeris-taxa hexasticha and intermedia: species or subspecies? - allozyme data (Diplopoda, Glomerida: Glomeridae). In: Enghoff, H. (Ed.), Many-legged animals - A collection of papers on Myriapoda and Onychophora - Entomologica scandinavia, 51 (Supplement), pp. 133–138.

Hoess, R. & Scholl, A. (1999a) Glomeris undulata Koch and Glomeris conspersa Koch are conspecific. Enzyme electrophoretic evidence and taxonomical consequences (Diplopoda: Glomeridae). Revue Suisse de Zoologie, 106 (3), 643–661.

Hoess, R. & Scholl, A. (1999b) The identity of Glomeris quadrifasciata C. L. Koch (Diplopoda: Glomeridae). Revue Suisse de Zoologie, 106 (4), 1013–1024.

Hoess, R. & Scholl, A. (2001) Allozyme and literature study of Glomeris guttata Risso, 1826, and G. connexa Koch, 1847, a case of taxonomic confusion (Diplopoda: Glomeridae). Zoologischer Anzeiger, 240 (1), 15–33.
http://dx.doi.org/10.1078/0044-5231-00003

Hoess, R. (2000) Bestimmungsschlüssel für die Glomeris-Arten Mitteleuropas und angrenzender Gebiete (Diplopoda: Glomeridae). Jahrbuch des Naturhistorischen Museums Bern, 13, 3–20.

Kime, R.D. (2000) Present knowledge of the distribution of European millipedes (Diplopoda). In: Wytwer, J. & Golovatch, S.I. (Eds.), Progress in Studies on Myriapoda and Onychophora. Fragmenta faunistica, Warszawa, 43 (Supplement 2000), pp. 281–294.

Kime, R.D. & Enghoff, H. (2011) Atlas of European Millipedes (Class Diplopoda). Volume 1. Orders Polyxenida, Glomerida, Platydesmida, Siphonocryptida, Polyzoniida, Callipodida, Polydesmida. Pensoft Publishers amd European Invertebrate Survey, Sofia-Moscow-Leiden, 282 pp.

Koch, C.L. (1847) System der Myriapoden mit den Verzeichnissen und Berichtigungen zu Deutschlands Crustaceen, Myriapoden und Arachniden. In: Panzer & Herrich-Schöffer, A. (Eds.), Kritische Revision der Insectenfauna Deutschlands. III. Bändchen, Regensburg, pp. 1–196.
http://dx.doi.org/10.5962/bhl.title.49866

Koch, C.L. (1863) Die Myriapoden. Getreu nach der Natur abgebildet und beschrieben. Bd. 1. Halle. 134 pp.

Latreille, PA. (1803) Histoire naturelle, générale et particulière des Crustacés et des Insectes. 3 + 7. (= Tom 95 + 99). Dufart, Paris, 467 pp.

Latzel, R. (1884) Die Myriopoden der Österreichisch-ungarischen Monarchie. Zweite Hälfte. Die Symphylen, Pauropoden und Diplopoden. Alfred Hölder, Wien, 414 pp.

Nei, M. & Kumar, S. (2000) Molecular Evolution and Phylogenetics. Oxford University Press, New York, 333 pp.

Preudhomme de Borre, A. (1884) Tentamen catalogi Glomeridarum hucusque descriptarum. Annales de la Societe entomologique de Belgique, 1884, 19–28.

Reip, H.S., Decker, P., Voigtländer, K., Spelda, J., Lindner, N. & Hannig, C. (2012) Seltene Myriapoden Deutschlands (Diplopoda, Chilopoda). Schubartiana, 5, 49–20.

Rothenbühler, H. (1899) Ein Beitrag zur Kenntnis der Myriapodenfauna der Schweiz. Revue Suisse de Zoologie, 6, 199–271.
http://dx.doi.org/10.5962/bhl.part.17668

Schubart, O. (1934) Tausendfüßler oder Myriapoda. I: Diplopoda. Die Tierwelt Deutschlands und der angrenzenden Meeresteile, 28, 1–318.

Spelda, J. (1991) Zur Faunistik und Systematik der Tausendfüßler (Myriapoda) Südwestdeutschlands. Jahreshefte der Gesellschaft für Naturkunde in Württemberg, 146, 211–232.

Spelda, J. (1998) Provisorische Rote Liste der in Baden-Württemberg gefährdeten Hundert- und Tausendfüßer (Myriapoda: Chilopoda, Diplopoda). Stand: August 1997. Rote Listen auf CD-ROM, 1–44.

Spelda, J. (1999) Verbreitungsmuster und Taxonomie der Chilopoda und Diplopoda Südwestdeutschlands. Diskriminanzanalytische Verfahren zur Trennung von Arten und Unterarten am Beispiel der Gattung Rhymogona Cook, 1896 (Diplopoda: Chordeumatida: Craspedosomatidae). Teil 2. Dissertation zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften der Universität Ulm, 1999, 1–324.

Spelda, J. (2006) Improvements in the knowledge of the myriapod fauna of southern Germany between 1988 and 2005 (Myriapoda: Chilopoda, Diplopoda, Pauropoda, Symphyla). Peckiana, 4 (2005), 101–129.

Spelda, J., Reip, H.S., Oliveira-Biener, U. & Melzer, R.R. (2011) Barcoding Fauna Bavarica: Myriapoda – a contribution to DNA sequence-based identifications of centipedes and millipedes (Chilopoda, Diplopoda). ZooKeys, 115, 123–139.
http://dx.doi.org/10.3897/zookeys.156.2176

Strasser, K. (1965) Über Diplopoden aus Kärnten und anderen Ostalpenländern. Carinthia II, 75, 127–142

Strasser, K. (1971) Catalogus Faunae jugoslaviae. III/5. Diplopoda. Ljubljana: Academia Scientarum et Artium Slovenica, 3, 3–50.

Strasser, K. & Minelli, A. (1984) Elenco dei diplopodi d'Italia - A checklist of Italian Diplopods. Societa Veneziana di Scienze Naturali Lavori, 9 (2), 193–212.

Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10, 512–526.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
http://dx.doi.org/10.1093/molbev/mst197

Verhoeff, K.W. (1894) Beiträge zur Diplopoden-Fauna der Schweiz. Berliner entomologische Zeitschrift, 38 (2), 281–296.
http://dx.doi.org/10.1002/mmnd.18940390214

Verhoeff, K.W. (1909) Über Diplopoden: Zur Kenntnis der Glomeriden. Zoologischer Anzeiger, 35 (4–5), 101–124.

Verhoeff, K.W. (1912) Zur Kenntnis einiger mitteleuropäischer Chilognathen und der Schläfenorgane der Plesiocerata. Sitzungsberichte der Gesellschaft naturforschender Freunde in Berlin, 1912 (8), 415–438.

Verhoeff, K.W. (1917) Zur Kenntnis der Zoogeographie Deutschlands, zugleich über Diplopoden namentlich Mitteldeutschlands und Beiträge für die biologische Beurteilung der Eiszeiten. Nova Acta. Abhandlungen der Kasiserlichen Leop.-Carol. Deutschen Akademie der Naturforscher, 103, 1–157.

Villers, J. (1789) Caroli Linnaei Entomologia, faunae Suecicae descriptionibus aucta Scopoli, Geoffroy, de Geer, Fabricii, Schrank. 4. Aptera. Sumptibus Piestre et Delamolliere, Lugduni, CCXIII + 556 pp., 1 tab., 1 t


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