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DOI: http://dx.doi.org/10.11646/zootaxa.4006.1.7

A new leaf-tailed gecko of the Uroplatus ebenaui group (Squamata: Gekkonidae) from Madagascar's central eastern rainforests

FANOMEZANA MIHAJA RATSOAVINA, FIADANANTSOA ANDRIANJA RANJANAHARISOA, FRANK GLAW, ACHILLE P. RASELIMANANA, AURÉLIEN MIRALLES, MIGUEL VENCES

Abstract


We describe a new leaf-tailed gecko species of the Uroplatus ebenaui group from the eastern central rainforests of Madagascar, which had previously been considered as a confirmed candidate species. Our description of Uroplatus fiera sp. nov. relies on integrating evidence from molecular and morphological characters and is based on newly collected material from two localities. A phylogenetic analysis based on multiple mitochondrial DNA fragments places the new species as sister to a lineage of uncertain status (Uroplatus ebenaui [Ca8]), and the clade consisting of these two lineages is sister to a further undescribed candidate species (U. ebenaui [Ca1]). This entire clade is sister to U. phantasticus plus another candidate species. The new species differs from these close relatives, and all other congenerics, by strong differences in DNA sequences of mitochondrial genes (>8.5% uncorrected p-distance in 16S rDNA to all nominal species of the genus) and lacks shared alleles with any of the nominal species in the nuclear CMOS gene. From its closest relatives the new species further differs in its much smaller tail size (relative to U. phantasticus), and a narrower tail, fewer supralabials, and more toe lamellae (relative to U. ebenaui [Ca1]). Morphologically the new species is most similar to U. ebenaui but differs in its larger body size and unpigmented oral mucosa. Given its distribution in central eastern Madagascar, with records from near Fierenana and Ambatovy, its range overlaps with that of U. phantasticus. Based on examination of the U. phantasticus holotype, we confirm that this latter has a blackish pigmented oral mucosa as do those specimens typically attributed to this nomen, thereby confirming its distinctness from U. fiera sp. nov., in which the mucosa is unpigmented.

 


Keywords


Reptilia, Uroplatus fiera sp. nov., Uroplatus ebenaui group, Taxonomy, Phylogeny, Gekkonidae, Fierenana, Madagascar

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References


Bandelt, H.-J., Forster, P. & Röhl, A. (1999) Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16, 37–48.
http://dx.doi.org/10.1093/oxfordjournals.molbev.a026036

Bauer, A.M. & Russell, A.P. (1989) A systematic review of the genus Uroplatus (Reptilia: Gekkonidae), with comments on its biology. Journal of Natural History, 23, 169–203.
http://dx.doi.org/10.1080/00222938900770101

Böhme, W. & Henkel, F.-W. (1995) Studien an Uroplatus II: Der Artenkomplex um Uroplatus ebenaui Boettger, 1879, mit Bemerkungen zu Uroplatus guentheri Mocquard, 1908. Herpetofauna, 17 (94), 11–23.

Boettger, O. (1879) Die Reptilien und Amphibien von Madagascar. Erster Nachtrag. Abhandlungen der Senckenbergischen naturforschenden Gesellschaft, 11 (1878), 269–282.

Boulenger, G.A. (1888) Description of new reptiles and batrachians from Madagascar. Annals and Magazine of Natural History, 6, 101–107.
http://dx.doi.org/10.1080/00222938809460688

Boumans, L., Vieites, D.R., Glaw, F. & Vences, M. (2007) Geographical patterns of deep mitochondrial differentiation in widespread Malagasy reptiles. Molecular Phylogenetics and Evolution, 45, 822–839.
http://dx.doi.org/10.1016/j.ympev.2007.05.028

Bruford, M.W., Hanotte, O., Brookfield, J.F.Y. & Burke, T. (1992) Single-locus and multilocus DNA fingerprint. In: Hoelzel, A.R. (Ed.), Molecular Genetic Analysis of Populations: A Practical Approach. IRL Press, Oxford, 225–270.

Glaw, F., Kosuch, J., Henkel, F.-W., Sound, P. & Böhme, W. (2006) Genetic and morphological variation of the leaf-tailed gecko Uroplatus fimbriatus from Madagascar, with description of a new giant species. Salamandra, 42, 129–144.

Glaw, F. & Vences, M. (2007) A Field Guide to the Amphibians and Reptiles of Madagascar. 3rd edition. Vences & Glaw Verlag, Cologne, 496 pp.

Greenbaum, E., Bauer, A.M., Jackman, T.R., Vences, M. & Glaw, F. (2007) A phylogeny of the enigmatic Madagascan geckos of the genus Uroplatus (Squamata: Gekkonidae). Zootaxa, 1493, 41–51.

IUCN [World Conservation Union] (2001) IUCN red list categories. Version 3.1. Gland, Cambridge: Species Survival Commission, IUCN.

Lees, D.C. (1996) The Perinet effect? Diversity gradients in an adaptive radiation of Madagascan butterflies (Satyrinae: Mycalesina) contrasted with other species-rich rainforest taxa. In: Lourenco, W.R. (Ed.), Biogeographie de Madagascar. ORSTOM, Paris, pp. 479–490.

Lees, D.C., Kremen, C. & Andriamampianina, L. (1999) A null model for species richness gradients: bounded range overlap of butterflies and other rainforest endemics in Madagascar. Biological Journal of the Linnean Society, 67, 529–584.
http://dx.doi.org/10.1111/j.1095-8312.1999.tb01945.x

Librado, P. & Rozas, J. (2009) DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451–1452.
http://dx.doi.org/10.1093/bioinformatics/btp187

Nagy, Z.T., Sonet, G., Glaw, F. & Vences, M. (2012) First large-scale DNA barcoding assessment of reptiles in the biodiversity hotspot of Madagascar, based on newly designed COI primers. PloS ONE, 7, e34506.
http://dx.doi.org/10.1371/journal.pone.0034506

Nussbaum, R.A. & Raxworthy, C.J. (1995) New Uroplatus Duméril (Reptilia: Squamata: Gekkonidae) of the ebenaui-group from the Anosy Mountains of southern Madagascar. Copeia, 1995 (1), 118–124.
http://dx.doi.org/10.2307/1446806

Nylander, J.A.A. (2002) MrModeltest v1.0b. Program distributed by the author. Available from: http://www.ebc.uu.se/systzoo/staff/nylander.html (accessed 7 July 2015)

Padial, J.M., Miralles, A., De la Riva, I. & Vences, M. (2010) The integrative future of taxonomy. Frontiers in Zoology, 7 (article 16), 1–14.
http://dx.doi.org/10.1186/1742-9994-7-16

Perl, R.G.B., Nagy, Z.T., Sonet, G., Glaw, F., Wollenberg, K.C. & Vences, M. (2014) DNA barcoding Madagascar’s amphibian fauna. Amphibia-Reptilia, 35, 197–206.
http://dx.doi.org/10.1163/15685381-00002942

Ratsoavina, F.M., Louis, E.E. Jr., Crottini, A., Randrianiaina, R.D., Glaw, F. & Vences, M. (2011) A new leaf tailed gecko species from northern Madagascar with a preliminary assessment of molecular and morphological variability in the Uroplatus ebenaui group. Zootaxa, 3022, 39–57.

Ratsoavina, F.M., Vences, M. & Louis, E.E. Jr. (2012) Phylogeny and phylogeography of the Malagasy leaf-tailed geckos in the Uroplatus ebenaui group. African Journal of Herpetology, 61, 143–158.
http://dx.doi.org/10.1080/21564574.2012.729761

Ratsoavina, F.M., Raminosoa, N.R., Louis, E.E. Jr., Raselimanana, A.P., Glaw, F. & Vences, M. (2013) An overview of Madagascar’s leaf tailed geckos (genus Uroplatus): species boundaries, candidate species and review of geographical distribution based on molecular data. Salamandra, 49, 115–148.

Raxworthy, C.J., Pearson, R.G., Zimkus, B.M., Reddy, S., Deo, A.J., Nussbaum, R.A. & Ingram, C.M. (2008) Continental speciation in the tropics: contrasting biogeographic patterns of divergence in the Uroplatus leaf-tailed gecko radiation of Madagascar. Journal of Zoology, 275, 423–440.
http://dx.doi.org/10.1111/j.1469-7998.2008.00460.x

Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
http://dx.doi.org/10.1093/bioinformatics/btg180

Stephens, M., Smith, N.J. & Donnelly, P. (2001) A new statistical method for haplotype reconstruction from population data. American Journal of Human Genetics, 68, 978–989.
http://dx.doi.org/10.1086/319501

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.
http://dx.doi.org/10.1093/molbev/msr121

Vences, M., Gehara, M., Köhler, J. & Glaw, F. (2012) Description of a new Malagasy treefrog (Boophis) occurring syntopically with its sister species, and a plea for studies on non-allopatric speciation in tropical amphibians. Amphibia-Reptilia, 33, 503–520.
http://dx.doi.org/10.1163/15685381-00002856

Vieites, D.R., Wollenberg, K.C., Andreone, F., Köhler, J., Glaw, F. & Vences, M. (2009) Vast underestimation of Madagascar’s biodiversity evidenced by an integrative amphibian inventory. Proceedings of the National Academy of Sciences of the United States of America, 106, 8267–8272.
http://dx.doi.org/10.1073/pnas.0810821106

Wilmé, L., Goodman, S.M. & Ganzhorn, J.U. (2006) Biogeographic evolution of Madagascar's microendemic biota. Science, 312, 1063–1065.
http://dx.doi.org/10.1126/science.1122806

Yoder, A.D. & Nowak, M.D. (2006) Has vicariance or dispersal been the predominant biogeographic force in Madagascar? Only time will tell. Annual Reviews of Ecology, Evolution, and Systematics, 37, 405–431.
http://dx.doi.org/10.1146/annurev.ecolsys.37.091305.110239


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