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NEW METHOD OF ESTIMATION OF THE RELATIVE AREA OF PERFORATIONS ON VALVES OF CENTRIC DIATOMS USING SEM IMAGES ON THE EXAMPLE OF MINIDISUS VODYANITSKIYI LYAKH ET BEDOSHVILI

Anton M. Lyakh, Yekaterina D. Bedoshvili, Olga V. Shikhat

Abstract


The diatoms interact with the environment through the siliceous frustule. The total area of frustule perforations determines the ability of diatom to exchange nutrients, gases and other matters. The aim of the present study was to estimate the area of perforations on the valve surface of a centric diatom. In the paper we describe a method for the estimation of the area of perforations on a diatom valve using SEM images. The method is tested on valves of centric diatom Minidiscus vodyanitskiyi Lyakh et Bedoshvili. The results show that the total area of cribral pores is less than 5% of the total valve area. This value is consistent with the relative perforation of land plants leaves, which is less than 3%. We hypothesize that such small valve area occupied by perforations is usual for many other centric diatom species. To verify this hypothesis additional researches are necessary.

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References


Bukhtiyarova, L.N. (2009) Frustule function and functional morphology of Bacillariophyta. Algologia, 19 (3), 321–331.

Bukhtiyarova, L.N. (2013) Morphology of new for Ukraine Bacillariophyta from the hydrotopes of right-bank forest-steepe. II. Species of Gomphonema Ehrenb. Modern Phytomorphology, 3, 231–240.

Bukhtiyarova, L.N. (2015) Classification of uniserial striae in Bacillariophyta with bipolar frustule. Algologia, 25 (2), 198–210.

De Stefano, L., Lamberti, A., Rotiroti L. & De Stefano, M. (2008) Interfacing the nanostructured biosilica microshells of the marine diatom Coscinodiscus wailesii with biological matter. Acta Biomaterialia, 2008, 4 (1), 126–130.

Hale, M. S. & Mitchell, J. G. (2001) Functional morphology of diatom frustule microstructures: hydrodynamic control of Brownian particle diffusion and advection. Aquatic Microbial Ecology, 24, 287–295.

Hein, M., Pedersen, M. F. & Sand-Jensen, K. (1995) Size-dependent mitrogen uptake in micro- and macroalgae. Marine Ecology Progress Series, 118, 247-253.

Lawson, T. & Blatt, M. (2014) Stomatal size, speed, and responsiveness impact on photosynthesis and water use efficiency. Plant Physiology, 2014, 164 (4), 1556–1570.

Luis, A. T., Hlubikova, D., Vache, V., Choquet, P., Hoffmann, L. & Ector, L. (2017) Atomic force microscopy (AFM) application to diatom study: review and perspectives. Journal of Applied Phycology, 29 (6), 2989–3001.

Losic, D., Pillar, R. J., Dilger, T., Mitchell, J. G. & Voelcker, N. H. (2007) Atomic force microscopy (AFM) characterization of the porous silica nanostructure of two centric diatoms. Journal of Porous Materials, 14 (1), 61–69.

Lyakh, A. M. (2013) The model of areolae distribution and surface area of pores located on the valves of centric diatoms Coscinodiscus Ehr. and Thalassiosira Cl. Modern Phytomorphology, 3, 213–218. (In Russian).

Lyakh, A. M. & Bedoshvili, Ye. D. (2018) Tiny diatoms Minidiscus vodyanitskiyi sp. nov. (Bacillariophyta) from the Sea of Azov and consideration of polygonal areolae pattern. Phytotaxa, 375 (2), 171–181.

Pahlow, M., Riebesell, U. & Wolf-Gladrow, D.A. (1997). Impact of cell shape and chain formation on nutrient acquisition by marine diatoms. Limnology and Oceanography, 42 (8), 1660–1672.

Pernsteiner, S. (2019) Ellipse by 5 points extension. Available from: http://pernsteiner.org/inkscape/ellipse_5pts. (April 4th, 2019).

Pickett-Heaps J., Schmid A.-M.M. & Edgar L.A. (1990) The cell biology of diatom valve formation. In: Round, F.E. & Chapman, D.J. (Eds.), Progress in phycological research. Biopress Ltd, Bristol, pp. 1–168.

Sicko-Goad, L., Stoermer, E. F. & Ladewski, B. G. (1977). A morphometric method for correcting phytoplankton cell volume estimates. Protoplasma, 93 (2–3), 147–163.


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