Proteolytic and biocatalytic activities of animal spleen extracts

Jambalsuren Bayarmaa, Dondog Purev

Abstract


Proteolytic activity and biocatalytic properties of spleen extracts from six animals were studied. Among tested spleens, extracts from the camel’s spleen showed the highest activity, followed by bovine, horse, caprine, porcine and ovine extracts. Also the effects of NaCl and (NH4)2SO4 on proteolytic activity was investigated. Spleen extracts contained heat stable acid protease with optimum pH of 2.6 and Topt at 40ºC. Thermal inactivation kinetics was described as second order model.


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ISSN 1684 (print edition), ISSN 2225-4994 (online edition)