DOI: http://dx.doi.org/10.22073/pja.v8i2.43363

Varroa destructor (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI

Zahra Hajializadeh, Mahdieh Asadi, Kamal Ahmadi, Asieh Balvasi

Abstract


A molecular characterization based on mitochondrial cytochrome oxidase subunit I gene was used to genotype central-southern Iranian populations of Varroa destructor for the first time. A 570 bp segement was amplified by Polymerase Chain Reaction and digested with two restriction enzymes. Digestion of PCR products with XhoI produced fragments of 300 and 270 bp in all samples, whereas digestion with SacI did not produce any digested fragments. Results suggest that all samples belong to the K haplotype previously described for V. destructor in the literature. A total of 22 sequences of Iranian samples (418bp) were registered for the first time in NCBI.

Keywords


Genetic diversity; haplotype; Mesostigmata; mitochondrial genome; molecular characterization.

Full Text:

PDF PDF/A

References


Anderson, D. L. & Fuchs, S. (1998) Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. Journal of Apicultural Research, 37: 69–78.

Anderson, D. L. & Trueman, J. W. H. (2000) Varroa jacobsoni (Acari: Varroidae) is more than one species. Experimental and Applied Acarology, 24: 165–189.

Dadgostar, Sh. & Nozari, J. (2018) Classical and geometric morphometric methods reveal differences between specimens of Varroa destructor (Mesostigmata: Varroidae) from seven provinces of Iran. Persian Journal of Acarology, 7(1): 51–60.

De la Rúa, P., Jaffé, R., Dall'Olio, R., Muñoz, I. & Serrano, J. (2009) Biodiversity, conservation and current threats to European honeybees. Apidologie, 40(3): 263–284.

Farjamfar, M., Saboori, A., González‑Cabrera, J. & Rodríguez, C.S.H. (2018) Genetic variability and pyrethroid susceptibility of the parasitic honey bee mite Varroa destructor (Acari: Varroidae) in Iran. Experimental and Applied Acarology, 76(1): 139–148.

Gajić, B., Radulović, Z., Stevanović, J., Kulisić, Z., Vucicević, M., Simeunović, P. & Stanimirović, Z. (2013) Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. Experimental and Applied Acarology, 61: 97–105.

Genersch, E., von der Ohe, W., Kaatz, H., Schroeder, A., Otten, C., Büchler, R., Berg, S., Ritter, W., Mühlen, W., Gisder, S., Meixner, M., Liebig, G. & Rosenkranz, P. (2010) The German bee monitoring project: a long term study to understand periodically high winter losses of honey bee colonies. Apidologie, 41: 332–352.

Hajializadeh, Z., Asadi, M. & Kavousi, H. (2018) First report of Varroa genotype in western Asia based on genotype identification of Iranian Varroa destructor populations (Mesostigmata: Varroidae) using RAPD marker. Systematic and Applied Acarology, 23(2): 199–205.

Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95–98.

Kelomey, A.E., Paraiso, A., Sina, H., Legout, H., Garnery, L. & Baba-Moussa, L. (2017) Genetic characterization of the honeybee ectoparasitic mite Varroa destructor from Benin (West Africa) using mitochondrial and microsatellite markers. Experimental and Applied Acarology, 72(1): 61–67.

Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35: 1547–1549.

Moshaverinia, A., Abedi, V. & Safaei, H. (2013) Mite infestation of honey bee (Apis mellifera) in apiaries of North East of Iran. Scientica Parasitologica, 14(1): 31–35.

Muñoz, I., Garrido-Bailón, E., Martín-Hernández, R., Meana, A., Higes, M. & De la Rúa, P. (2008) Genetic profile of Varroa destructor infesting Apis mellifera iberiensis colonies. Journal of Apicultural Research, 47(4): 310–313.

Navajas, M., Anderson, D.L., de Guzman, L.I., Huang, Z.Y., Clement, J., Zhou, T. & Le Conte, Y. (2010) New Asian types of Varroa destructor: a potential new threat for world apiculture. Apidologie, 41: 181–193.

Sanford, M.T. (2001) Introduction, spread and economic impact of Varroa mites in North America. In: Webster, T.C., Delaplane, K.S. (Eds.), Mites of the honey bee. Dadant & Sons, Mansfield, OH, pp. 149–162.

Shahrouzi, R. (2012) Three decades of living with Varroa in Iran. available at: http://www. apiservices.biz/en/articles/ (Accessed on 10 November 2018).

Solignac, M., Cornuet, J. M., Vautrin, D., Le Conte, Y., Anderson, D., Evans, J., Cros-Arteil, S. & Navajas, M. (2005) The invasive Korea and Japan types of Varroa destructor, ectoparasitic mites of the Western honey bee (Apis mellifera), are two partly isolated clones. Proceedings of the Royal Society B: Biological Sciences, 272(1561): 411–419.

Strapazzon, R., Carneiro, F. E., Guerra Jr, J.V.C. & Moretto, G. (2009) Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the state of Santa Catarina, Brazil. Genetics and Molecular Research, 8(3): 990–997.


Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Persian Journal of Acarology

License URL: http://creativecommons.org/licenses/by-nc-nd/2.0/fr/deed.en_GB