Open Access Open Access  Restricted Access Subscription Access


DOI: http://dx.doi.org/10.11158/saa.23.2.1

First report of Varroa genotype in western Asia based on genotype identification of Iranian Varroa destructor populations (Mesostigmata: Varroidae) using RAPD marker

Zahra Hajializadeh, Mahdieh Asadi, Hamidreza Kavousi

Abstract


Random amplified polymorphic DNA (RAPD) technique was used to the analysis of the haplotypes of Varroa destrcuctor in southern half of Iran. Eight Varroa populations were collected and five RAPD primers were used to determine their genotype. All samples showed the K pattern genotype. Furthermore 30 loci were amplified; from which 29 were polymorphic (96.67%) and 1 was monomorphic (3.33%). This is the first molecular genetic study of V. destructor in Iran. Detailed investigations at DNA sequence level are needed to characterize genetic structure of V. destructor populations in Iran.


Keywords


Varroa destructor, genotype, RAPD, Korean type, western Asia, Iran.

References


Akimov, I.A., Benedyk, S.V., Berezovskaya, O.P. & Andsidorenk, A.P. (2004) RAPD analysis of intraspecific genetic variability of the mite Varroa destructor (Parasitiformes, Varroidae) in Ukraine. Acarina, 12(2), 113–119.

Anderson, D.L. (2000) Variation in the parasitic bee mite Varroa jacobsoni Oud. Apidologie, 31(2), 281–292.

https://doi.org/10.1051/apido:2000122

Anderson, D.L. & Fuchs, S. (1998) Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. Journal of Apicultural Research, 37, 69–78.

http://doi.org/10.1080/00218839.1998.11100957

Anderson, D.L. & Trueman, J.W. (2000) Varroa jacobsoni (Acari: Varroidae) is more than one species. Experimental and Applied Acarology, 24, 165–189.

https://doi.org/10.1051/apido:2000122

Anon (1999) Statistica 99 Edition. Statistica for Windows (Computer program manual). Kernel release 5.5 A. Tulsa, OK. StatSoft Inc.

Awad, N.S., Allam, S.F.M., Rizk, M.A., Hassan, M.F. & Zaki, A.Y. (2010) Fingerprinting and assessment of genetic variability of Varroa destructor in Egypt. Journal of Apicultural Research, 49(3), 251–256.

http://doi.org/10.3896/IBRA.1.49.3.04

Baker, A.R. (2010) The parasitic mites of honey bees - past, present and future. Journal of Entomological Research, 1(4), 1–7.

Boecking, O. & Genersch, E. (2008) Varroasis the ongoing crisis in bee keeping. Journal of Consumer Protection and Food Safety, 3(2), 221–228.

http://dx.doi.org/10.1007/s00003-008-0331-y

Bokaie, S., Sharifi, L. & Mehrabadi, M. (2014) Prevalence and Epizootical Aspects of Varroasis in Golestan Province, Northern Iran. Journal of Arthropod-Borne Diseases, 8(1), 102–107.

Correˆa-Marques, M.H., De Jong, D., Rosenkranz, P. & Goncalves, L.S. (2002) Varroa-tolerant Italian honey bees introduced from Brazil were not more efficient in defending themselves against the mite Varroa destructor than Carniolan bees in Germany. Genetic and molecular research, 1, 153–158.

De Guzman, L.I. & Delfinado-Baker, M. (1996) A new species of Varroa (Acari: Varroidae) associated with Apis koschevnikovi (Apidae: Hymenoptera) in Borneo. International Journal of Acarology, 22(1), 23–27.

http://doi.org/10.1080/01647959608684077

De Guzman, L.I. & Rinderer, T.E. (1999) Identification and comparison of Varroa species infesting honey bees. Apidologie, 30(2-3), 85-95.

De Guzman, L.I., Rinderer, T.E. & Stelzer, J.A. (1997) DNA evidence of the origin of Varroa jacobsoni Oudemans in the Americas. Biochemical Genetics, 35, 327–335.

https://doi.org/10.1023/A:1021821821728

De Guzman, L.I., Rinderer, T.E. & Stelzer, J.A. (1999) Occurrence of two genotypes of Varroa jacobsoni Oud. in North America. Apidologie, 30, 31–36.

https://doi.org/10.1051/apido:19990104

De Jong, D. (1984) Reproduction of Varroa jacobsoni Oud. in Europe, the middle East and tropical South America. Journal of Applied Entomology, 98, 55–57.

De Jong, D., Goncalves, L.S. & Morse, R.A. (1984) Dependence on climate of the virulence of Varroa jacobsoni. Bee World, 65, 117–121.

https://doi.org/10.1080/0005772X.1984.11098789

De la Rúa, P., Jaffé, R., Dall'Olio, R., Muñoz, I. & Serrano, J. (2009) Biodiversity, conservation and current threats to European honeybees. Apidologie, 40(3), 263–284.

https://doi.org/10.1051/apido/2009027

Garedew, A., Schmolz, E. & Lamprecht, I. (2004) The energy and nutritional demand of the parasitic life of the mite Varroa destructor. Apidologie, 35, 419–430.

https://doi.org/10.1051/apido:2004032

Guerra, J.C.V., Issa, M.R.C., Carneiro, F.E., Strapazzon, R. & Moretto, G. (2010) RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas. Genetic and molecular research, 9(1), 303–308.

Kraus, B. & Hunt, G. (1995) Differentiation of Varroa jacobsoni Oud. Populations by random amplification of polymorphic DNA (RAPD). Apidologie, 26, 283–290.

https://doi.org/10.1051/apido:19950402

Liu, K. & Muse, S.V. (2005) Power Marker: an integrated analysis environment for genetic marker data. Bioinformatics, 21(9), 2128–2129.

https://doi.org/10.1093/bioinformatics/bti282

Maadinia, M., Shabestari, B., Mahmoudi, R., Kaboudari, A. & Rahimi Pir-Mahalleh, S.F. (2015) Evaluation of Varroa Mites in the Apiaries from Iran. International Journal of Food Nutrition and Safety, 6(2), 74–81.

Maniatis, T., Fritsch, E.F. & Sambrook, J. (1983) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, New York. Biochemistry and Molecular Biology Education, 11(2), pp. 82.

Moretto, G., Goncalves, L.S. & De Jong, D. (1991) The effects of climate and bee race on Varroa jacobsoni Oud. infestations in Brazil. Apidologie, 22, 197–203.

https://doi.org/10.1051/apido:19910303

Moshaverinia, A., Abedi, V. & Safaei, H. (2013) Mite infestation of honeybee (Apis mellifera) in apiaries of North East of Iran. Scientica parasitologica, 14(1), 31–35.

Peakall, R. & Smouse, P.E. (2006) GENALEX 6.4: Genetic analysis in Excel: population genetic software for teaching and research. Molecular Ecology Notes, 6, 288–295.

http://dx.doi.org/10.1111/j.1471-8286.2005.01155.x

Rahimi, A., Mirmoayedi, A. & Khanjani, M. (2014) Dispersion type of Varroa mite (Acari, Varroidae) among the bee hives in kurdistan province, Iran. International Journal of Farming and Allied Sciences, 3(3), 255–259.

Rahmani, H., Kamali, K., SAboori, A. & Nowzari, J. (2006) Report and survey of morphometric characteristics of Varroa destructor (Acari: Varroidae) collected from honey bees in Tehran province, Iran. Journal of Agricultural Science and Technology, 8, 351–355.

Rosenkranz, P., Aumeier, P. & Ziegelmann, B. (2010) Biology and control of Varroa destructor. Journal of Invertebrate Pathology, 103, 96–119.

http://doi.org/10.1016/j.jip.2009.07.016

Solignac, M., Vautrin, D., Pizzo, A., Navajas, M., Le Conte, Y. & Cornuet, J.M. (2003) Characterization of microsatellite markers for the apicultural pest Varroa destructor (Acari: Varroidae) and its relatives. Molecular Ecology Resources, 3(4), 556–559.

http://dx.doi.org/10.1046/j.1471-8286.2003.00510.x

Webster, T.C. & Delaplane, K.S. (2001) Mites of the Honey Bee. Dadant and Sons, Inc., Hamilton, Illinois, 280 pp.

Welsh, J. & Mcclelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acid Research, 18, 7213–7218.

https://doi.org/10.1093/nar/18.24.7213

Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. & Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research, 18, 6531–6535.

https://doi.org/10.1093/nar/18.22.6531

Yeh, F.C., Yang, R.C. & Boyle, T. (1999) POPGENE version 1.32: Microsoft Windows–based freeware for population genetic analysis, quick user guide. Center for International Forestry Research, University of Alberta, Edmonton, Alberta, Canada.


Refbacks

  • There are currently no refbacks.


An international journal of the Systematic and Applied Acarology Society

ISSN 1362-1971