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Evaluation of Dermatophagoides pteronyssinus (Trouessart) and D. farinae Hughes (Acari: Pyroglyphidae) sensitivity in patients with allergic rhinitis: a comparative study

Erhan ZEYTUN, Salih DOĞAN, Edhem Ünver, Fatih ÖZÇÜÇEK


This study was conducted to determine the sensitivity to Dermatophagoides pteronyssinus (Trouessart) and D. farinae Hughes with skin prick tests (SPT) and serologic tests in patients with allergic rhinitis (AR), and to specifically search for those mites in homes of patients. A total of 51 participants, (23 patients and 28 controls) were utilized. Skin-prick tests with D. pteronyssinus and D. farinae allergens were performed on all participants, and serum levels of allergen-specific lgE and total IgE were also measured. Dust samples were collected from homes of all participants and examined under a stereo microscope. 977 D. pteronyssinus (mean 44.4/g) and 24 D. farinae (mean 4.0/g) were isolated from the homes of patients, whereas 35 D. pteronyssinus (mean 4.4/g), and four D. farinae (mean 2.0/g) were isolated from the homes of the controls. Patients with D. pteronyssinus in their homes had 95.5% sensitivity to the species according to SPT and 27.3 according to IgE. Patients with D. farinae in their homes had 83.3% sensitivity to the species according to SPT, and 50% according to IgE. Dermatophagoides pteronyssinus sensitivity in the controls was detected as 12.5% according to SPT; however, D. farinae sensitivity was not detected according to both SPT and mite-specific IgE. Differences between patients and controls utilizing SPT results was statistically significant, but not when using mite-specific IgE results. As a result, it was determined that patients with AR in Erzincan province were sensitized to D. pteronyssinus and D. farinae, and that their homes contained those species. It may be helpful to consider these findings in clinical assessment of patients with AR, and also in treatment utilizing immunotherapy techniques.


Allergic rhinitis, allergens, Dermatophagoides, house dust mite, immunotherapy


Akdemir, C. & Yılmaz, S. (2009) Sensitization to house-dust mite and mite fauna in selected children's homes in Kutahya, Turkey. The Turkish Journal of Pediatrics, 51, 232–237.

Akkaya, A., Ünlü, M. & Uygun, N. (1995) Evoluation of prick test positivity and total IgE levels in cases with allergic asthma and allergic rhinitis in Isparta district. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi, 2, 29–32. (article in Turkish with an abstract in English)

Al-Tamemi, S.H., Al-Shidhani, A.N., Al-Abri, R.K., Jothi, B., Al-Rawas, O.A. & Al-Riyami, B.M. (2008) The pattern of sensitisation to inhalant allergens in Omani patients with asthma, allergic rhinitis and rhinoconjunctivitis. Sultan Qaboos University Medical Journal, 8, 319–324.

Atambay, M., Aycan, Ö.M., Yoloğlu, S., Karaman, U. & Daldal, N. (2006) The relationship between the skin allergy test and house dust mites. Turkish Journal of Parasitology, 30, 327–329. (article in Turkish with an abstract in English)

Aykut, M., Erman, K. & Doğan, S. (2013a) Seasonal changes of house dust mite population in Bitlis and Muş provinces of Turkey. Turkish Journal of Parasitology, 37, 113–117.

Aykut, M., Erman, K., Doğan, S. & Ayyıldız, N. (2013b) House dust mites of Bitlis and Muş provinces. Turkish Bulletin of Entomology, 3, 169–177. (article in Turkish with an abstract in English)

Aykut, M., Erman, K. & Doğan, S. (2013c) Prevalence of house dust mites in houses, hotels and student dormitories of Diyarbakır province. Dicle University Institute of Natural and Applied Science Journal, 2(1), 41–47. (article in Turkish with an abstract in English)

Aykut, M., Erman, K. & Doğan, S. (2016) Variability in population density of house dust mites of Bitlis and Mus, Turkey. Journal of Medical Entemology, 53(3), 513–518.

Boquete, M., Iraola, V., Fernandez-Caldas, E., Villaroel, L.A., Carballada, F.J., de la Cuesta, C.G., Lopez-Rico, M.R., Orjales, R.N., Parra, A., Soto-Mera, M.T., Varela, S. & Vidal, C. (2006) House dust mite species and allergen levels in Galicia, Spain: a cross-sectional, multicenter, comparative study. Journal of Investigational Allergology and Clinical Immunology, 16, 169–176.

Bousquet, J., Khaltaev, N., Cruz A.A., Denburg, J., Fokkens, W., Togias, A., Zuberbier, T., Beana-Cagnani, C., Cononica, W.G., van Well, C., Agache, I., Ait-Khaled, N., Bachert, C., Blaiss, M., Bonini, S., Boulet, L.P., Bousquet, P.J., Camargos, P., Carlsen, K.H., Custovic, A., Chen, Y., Dahl, R., Demoly, P., Douagui, H., Durham, S., van Wijk, R.G., Kalayci, Ö., Kaliner, M., Kim, Y.Y., Kowalski, M.L., Kuna, P., Lemiere, C., Lan, L.T.T., Li, J., Lockey, R., Mavale, S., Meltzer, E.O., Mohammad, Y., Mullol, J., Naclerio, R., O’Hehir, R., Ohta, K., Ouedraogo, S., Palkonen, S., Papadopoulos, N., Passalacqua, G., Pawankar, R., Popov, T., Rabe, K., Rosado-Pinto, J., Scadding, G., Simons, F.E.R., Toskala, E., Valovirta, E., van Cauwenberge, P., Wang, D.Y., Wickman, M., Yawn, B., Yorgancioglu, A., Yusuf, O. & Zar, H. (2008) ARIA (Allergic rhinitis and its impact on asthma) 2008 update (In collaboration with the World Health Organization, GA2LEN and AllerGen). Allergy, 86, 8–134.

Calderon, M.A., Linneberg, A., Kleine-Tebbe, J., de Blay, F., de Rojas D.H.F., Virchow, J.C. & Demoly, P. (2015) Respiratory allergy caused by house dust mites: What do we really know? Journal of Allergy and Clinical Immunology, 136, 38–48.

Calvo, M., Fernandez-Caldas, E., Arellano, P., Marin, F., Carnes, J. & Hormaechea, A. (2005) Mite allergen exposure, sensitisation and clinical symptoms in Valdivia, Chile. Journal of Investigational Allergology and Clinical Immunology, 15, 189–196.

Chew, G.L., Reardon, A.M., Correa, J.C., Young, M., Acosta, L., Mellins, R., Chew, F.T. & Perzanowski, M.S. (2009) Mite sensitization among Latina women in New York, where dust-mite allergen levels are typically low. Indoor Air, 19, 193–197.

Çiftci, İ.H., Çetinkaya, Z., Aktepe, O.C., Kıyıldı, N., Aycan, O.M., Atambay, M. & Altındiş, M. (2004) The relation between house dust allergens and specific IgE. The Medical Journal of Kocatepe, 5, 29–32. (article in Turkish with an abstract in English)

Colloff, M.-J. (2009) Dust mites. New Zealand and Australia, Csiro Publishing, 592 pp.

Güleğen, E., Girişgin, O., Kütükoğlu, F., Girişgin, A.O. & Coşkun, S.Z. (2005) Mite species found in house dust in houses in Bursa. Turkish Journal of Parasitology, 29, 185–187. (article in Turkish with an abstract in English)

Harmancı, E., Us, T., Özdemir, N., Akgün, Y., Aydınlı, A. & Mutlu, S. (2000) The relationship between skin prick tests and serum specific IgE which is determined by chemiluminescence method in the diagnosis of respiratory system allergies. The Turkish Respiratory Journal, 2, 31–35. (article in Turkish with an abstract in English)

Ho, T.M., Murad, S., Kesavapillai, R. & Singaram, S.P. (1995) Prevalence of allergy to some inhalants among rhinitis patients in Malaysia. Asian Pacific Journal of Allergy and Immunology, 13, 11–16.

Kalpaklıoğlu, A.F., Emekci, M., Ferizli, A.G. & Mısırlıgil, Z. (2004). A survey of acarofauna in Turkey: comparison of seven different geographic regions. Allergy and Asthma Proceedings, 25, 185–190.

Kim, H.S., Kang, S.H., Won, S., Lee, E.K., Chun, Y.H., Yoon, J.S., Kim, H.H. & Kim, J.T. (2015) Immunoglobulin E to allergen components of house dust mite in Korean children with allergic disease. Asia Pacific Allergy, 5, 156–162.

Öztürk, O., Tokmak, A., Güçlü, E., Yıldızbaş, S. & Gültekin, E. (2005) Skin prick test results of patients with allergic rhinitis in Düzce. Düzce Medical Journal, 1, 11–14. (article in Turkish with an abstract in English)

Pagan, J.A., Huertas, A.J., Iraola, V., Pinto, H., Martinez, R., Ramirez, M., Martos, M.D. & Carnes, J. (2012) Mite exposure in a Spanish Mediterranean region. Allergologia et Immunopathologia, 40, 92–99.

Pumhirun, P., Towiwat, P. & Mahakit, P. (1997) Aeroallergen sensitivity of Thai patients with allergic rhinitis. Asian Pacific Journal of Allergy and Immunology, 15, 183–185.

Sanchez-Borges, M., Fernandez-Caldas, E., Capriles-Hulett, A. & Caballero-Fonseca, F. (2014) Mite hypersensitivity in patients with rhinitis and rhinosinusitis living in a tropical environment. Allergologia et Immunopathologia, 42, 120–126.

Seedat, R.Y., Claassen, J., Claassen, A.J. & Joubert, G. (2010) Mite and cockroach sensitisation in patients with allergic rhinitis in the Free State. South African Medical Journal, 100, 160–163.

Somorin, A.O., Hunponu-Wusu, O.O., Mumcuoğlu, K.Y. & Heiner, D.C. (1978) Mite allergy in Nigerians: studies on house dust mites in houses of allergic patients in Lagos. Irish Journal of Medical Science, 147, 26–30.

Spieksma, F.T. (1997) Domestic mites from an acarologic perspective. Allergy, 52, 360–368.

Valmonte, G.R., Cauyan, G.A. & Ramos, J.D.A. (2012) IgE cross-reactivity between house dust mite allergens and Ascaris lumbricoides antigens. Asia Pacific Allergy, 2, 35–44.

Vartiainen, E., Petays, T., Haahtela, T., Jousilahti, P. & Pekkanen, J. (2002) Allergic diseases, skin prick test responses, and IgE levels in North Karelia, Finland, and the Republic of Karelia, Russia. Journal of Allergy and Clinical Immunology, 109, 643–648.

Vidal-Quist J.C., Ortego, F., Lombardero, M., Castanera, P. & Hernandez-Crespo, P. (2015) Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions. Medical and Veterinary Entomology, 29, 137–146.

Yasan, H., Aynalı, G., Akkuş, O., Doğru, H., Özkan, M. & Sahin, M. (2006) Variaton and symptomatic correlation of allergen profile responsible for allergic rhinitis. KBB Forum, 5, 158–160. (article in Turkish with an abstract in English)

Yılmaz, N., Can, D., Asilsoy, S. & Gülle, S. (2009) The diagnostic value of specific IgE in allergic diseases. Asthma Allergy Immunology, 7, 111–117. (article in Turkish with an abstract in English)

Zeytun, E., Doğan, S., Aykut, M., Özçiçek, F., Ünver, E. & Özçiçek, A. (2015) House dust mites in Erzincan province. Turkish Journal of Parasitology, 39, 124–130.

Zeytun, E., Doğan, S., Özçiçek, F., Ünver, E. & Dilkaraoğlu, S. (2016) Comparison of living and bedrooms in terms of house dust mites in the province of Erzincan, Turkey. Journal of Medical Entemology, 53(1), 26–30.

Zeytun, E., Doğan, S., Özçiçek, F. & Ünver, E. (2017) Sensitivity to house dust mites allergens in patients with allergic asthma in Erzincan province, Turkey. Turkish Journal of Parasitology, 41, 34–41.

Zhang, Z.-Q., Fan, Q.-H., Pesic, V., Smit, H., Bochkov, A.V., Khaustov, A.A., Baker, A., Wohltmann, A., Wen, T., Amrine, J.W., Beron, P., Lin, J., Gabrys, G. & Husband, R. (2011) Order Trombidiformes Reuter, 1909. In: Zhang, Z.-Q. (Ed.) Animal biodiversity: An outline of higher-level classification and survey of taxonomic richness. Zootaxa, 3148, 129–138.

Zhang, C., Li, J., Lai, X., Zheng, Y., Gjesing, B., Spangford, M.D. & Zhong, N. (2012) House dust mite and storage mite IgE reactivity in allergic patients from Guangzhou, China. Asian Pacific Journal of Allergy and Immunology, 30, 294–300.


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